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Literature summary extracted from

  • Gunzler, V.; Brocks, D.; Henke, S.; Myllylä, R.; Geiger, R.; Kivirikko, K.I.
    Syncatalytic inactivation of prolyl 4-hydroxylase by synthetic peptides containing the unphysiologic amino acid 5-oxaproline (1988), J. Biol. Chem., 263, 19498-19504.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 bovine serum albumin activation Gallus gallus
1.14.11.2 bovine serum albumin activation Homo sapiens
1.14.11.2 catalase activation Gallus gallus
1.14.11.2 catalase activation Homo sapiens
1.14.11.2 dithiothreitol activation Gallus gallus
1.14.11.2 dithiothreitol activation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 benzyloxycarbonyl-Phe-Opr-Gly-benzyl ester 50% inactivation in 1 h at 0.0008 mM, the most effective inhibitor within oxaproline peptides Gallus gallus
1.14.11.2 benzyloxycarbonyl-Phe-Opr-Gly-benzyl ester 50% inactivation in 1 h at 0.0008 mM, the most effective inhibitor within oxaproline peptides Homo sapiens
1.14.11.2 additional information synthetic peptides containing the non-physiologic amino acid 5-oxaproline in the sequence R1-Xaa-oxaproline-Gly-OR2 are specific syncatalytic inactivators, noncompetitive inhibition with respect to peptide substrate and ascorbate, compounds with aromatic substituents R1 and R2 are particularly effective when compared with those with an aliphatic group, inactivation is only observed in the presence of Fe2+ and 2-oxoglutarate Gallus gallus
1.14.11.2 additional information synthetic peptides containing the non-physiologic amino acid 5-oxaproline in the sequence R1-Xaa-oxaproline-Gly-OR2 are specific syncatalytic inactivators, noncompetitive inhibition with respect to peptide substrate and ascorbate, compounds with aromatic substituents R1 and R2 are particularly effective when compared with those with an aliphatic group, inactivation is only observed in the presence of Fe2+ and 2-oxoglutarate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 additional information
-
 additional information
-
 Gallus gallus
1.14.11.2 additional information
-
 additional information
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+
-
 Gallus gallus
1.14.11.2 Fe2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Gallus gallus
-
-
-
1.14.11.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.2 using ammonium sulfate fractionation, affinity chromatography on poly(L-proline) coupled to Sepharose 4B, DEAE-cellulose chromatography, and gel filtration Gallus gallus
1.14.11.2 using ammonium sulfate fractionation, affinity chromatography on poly(L-proline) coupled to Sepharose 4B, DEAE-cellulose chromatography, and gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.2 embryo
-
 Gallus gallus
-
1.14.11.2 placenta
-
 Homo sapiens
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 37
-
 assay at Gallus gallus
1.14.11.2 37
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 ascorbate
-
 Gallus gallus
1.14.11.2 ascorbate
-
 Homo sapiens