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Literature summary extracted from

  • Britsch, L.; Grisebach, H.
    Purification and characterization of (2S)-flavanone 3-hydroxylase from Petunia hybrida (1986), Eur. J. Biochem., 156, 569-577.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.9 ascorbate required Petunia x hybrida
1.14.11.9 catalase stimulates Petunia x hybrida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.9 (+)-dihydrokaempferol product inhibition Petunia x hybrida
1.14.11.9 3-Bromo-2-oxoglutarate 1 mM 92% inhibition Petunia x hybrida
1.14.11.9 Cu2+
-
 Petunia x hybrida
1.14.11.9 diethyldithiocarbamate 2 mM, 81% inhibition Petunia x hybrida
1.14.11.9 EDTA 1 mM, 21% inhibition Petunia x hybrida
1.14.11.9 Fe3+ 1 mM, 56% inhibition Petunia x hybrida
1.14.11.9 KCN 1 mM, 11% inhibition Petunia x hybrida
1.14.11.9 p-chloromercuribenzoate 1 mM, 91% inhibition Petunia x hybrida
1.14.11.9 Pyridine-2,4-dicarboxylate 0.002 mM, 50% inhibition Petunia x hybrida
1.14.11.9 Pyridine-2,5-dicarboxylate 0.01 mM, 40% inhibition Petunia x hybrida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.9 additional information
-
 additional information varying Km-values, depending on the purification procedure Petunia x hybrida
1.14.11.9 0.0056
-
 (2S)-naringenin
-
 Petunia x hybrida
1.14.11.9 0.012
-
 (2S)-eriodictyol
-
 Petunia x hybrida
1.14.11.9 0.02
-
 2-oxoglutarate reaction with naringenin Petunia x hybrida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.9 Co2+ can partially replace Fe2+ Petunia x hybrida
1.14.11.9 Fe2+ required Petunia x hybrida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.9 35000
-
 x * 35000 + x * 37000, two-dimensional SDS-PAGE Petunia x hybrida
1.14.11.9 37000
-
 x * 35000 + x * 37000, two-dimensional SDS-PAGE Petunia x hybrida
1.14.11.9 74000
-
 gel filtration Petunia x hybrida

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.9 Petunia x hybrida
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.14.11.9 partially stabilized under anaerobic conditions in presence of ascorbate Petunia x hybrida

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.9
-
 Petunia x hybrida

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.9 additional information
-
-
 Petunia x hybrida

Storage Stability

EC Number Storage Stability Organism
1.14.11.9 -70°C, in presence of 20 mM ascorbate, stable for more than 6 months Petunia x hybrida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.9 (2S)-naringenin + 2-oxoglutarate + O2
-
 Petunia x hybrida (2R,3R)-dihydrokaempferol + succinate + CO2
-
 ?
1.14.11.9 eriodictyol + 2-oxoglutarate + O2 (2S)-eriodictyol Petunia x hybrida (2R,3R)-dihydroquercetin + succinate + CO2
-
 ?
1.14.11.9 additional information no activity with (2R)-naringenin and 5,7,3',4',5'-pentahydroxyflavanone Petunia x hybrida ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.9 ? x * 35000 + x * 37000, two-dimensional SDS-PAGE Petunia x hybrida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.9 8.5
-
-
 Petunia x hybrida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.9 0.0012
-
 Pyridine 2,4-dicarboxylate
-
 Petunia x hybrida
1.14.11.9 0.04
-
 Pyridine 2,5-dicarboxylate
-
 Petunia x hybrida