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Literature summary extracted from

  • Larkin, M.J.; Allen, C.C.R.; Kulakov, L.A.; Lipscomb, D.A.
    Purification and characterization of a novel naphthalene dioxygenase from Rhodococcus sp. strain NCIMB12038 (1999), J. Bacteriol., 181, 6200-6204.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.12 Fe2+ terminal dioxygenase ISPNAR may be a Rieske-type iron-sulfur protein containing 2.4 g-atoms of iron and 2.1 g-atoms of sulfur per alpha,beta subunit Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.12 23000
-
 alpha2beta2, 2 * 55000 + 2 * 23000, SDS-PAGE Rhodococcus sp.
1.14.12.12 55000
-
 alpha2beta2, 2 * 55000 + 2 * 23000, SDS-PAGE Rhodococcus sp.
1.14.12.12 155000
-
 terminal dioxygenase ISPNAR, gel filtration Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.12 Rhodococcus sp.
-
-
-
1.14.12.12 Rhodococcus sp. NCIMB12038
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.12 Blue Sepharose, Q-Sepharose, Superose 12, purification of component B, the terminal oxygenase ISPNAR Rhodococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.12 0.006
-
 activity of terminal dioxygenase ISPNAR Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.12 naphthalene + NAD(P)H + O2
-
 Rhodococcus sp. cis-(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD(P)+
-
 ?
1.14.12.12 naphthalene + NAD(P)H + O2
-
 Rhodococcus sp. NCIMB12038 cis-(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.12 More X-ray structure data from Pseudomonas sp. 9861-4 suggest an alpha3,beta3 hexameric structure Rhodococcus sp.
1.14.12.12 tetramer alpha2beta2, 2 * 55000 + 2 * 23000, SDS-PAGE Rhodococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.12 FAD stimulates activity of terminal oxygenase ISPNAR Rhodococcus sp.
1.14.12.12 FMN stimulates activity of terminal oxygenase ISPNAR Rhodococcus sp.
1.14.12.12 NADH
-
 Rhodococcus sp.
1.14.12.12 NADPH
-
 Rhodococcus sp.