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Literature summary extracted from

  • Fava, F.; Baldoni, F.; Marchetti, L.
    2-Chlorobenzoic acid and 2,5-dichlorobenzoic acid metabolism by crude extracts of Pseudomonas sp. CPE2 strain (1996), Lett. Appl. Microbiol., 22, 275-279.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.12.13 FAD slight inhibition Pseudomonas sp.
1.14.12.13 Fe2+ slight inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.13 0.063 0.079 2,5-dichlorobenzoate cells grown on 2-chlorobenzoate or 2,5-dichlorobenzoate Pseudomonas sp.
1.14.12.13 0.078 0.117 2-Chlorobenzoate cells grown on 2-chlorobenzoate or 2,5-dichlorobenzoate Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.13 Fe2+ required, ability to retain the endogenous Fe2+ during the crude extract perparation Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.13 2,5-dichlorobenzoate + NADH + O2 Pseudomonas sp. involved in the metabolism of 2,5-dichlorobenzoate 4-chlorocatechol + NAD+ + Cl- + CO2
-
 ?
1.14.12.13 2,5-dichlorobenzoate + NADH + O2 Pseudomonas sp. CPE2 involved in the metabolism of 2,5-dichlorobenzoate 4-chlorocatechol + NAD+ + Cl- + CO2
-
 ?
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2 Pseudomonas sp. involved in the metabolism of 2-chlorobenzoate catechol + chloride + NAD+ + CO2
-
 ?
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2 Pseudomonas sp. CPE2 involved in the metabolism of 2-chlorobenzoate catechol + chloride + NAD+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.13 Pseudomonas sp.
-
 CPE2
-
1.14.12.13 Pseudomonas sp. CPE2
-
 CPE2
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.13 additional information
-
 activity is similarly influenced by pH, temperature, concentration of oxygen, protein, Fe2+, FAD and NADH in assay medium independent if the cells are grown on 2-chlorobenzoate or 2,5-dichlorobenzoate Pseudomonas sp.
1.14.12.13 0.018 0.023 cells grown on 2-clorobenzoate or 2,5-dichlorobenzoate, enzyme activity for the conversion of both substrates Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.13 2,5-dichlorobenzoate + NADH + O2
-
 Pseudomonas sp. 4-chlorocatechol + chloride + NAD+ + CO2
-
 ?
1.14.12.13 2,5-dichlorobenzoate + NADH + O2
-
 Pseudomonas sp. CPE2 4-chlorocatechol + chloride + NAD+ + CO2
-
 ?
1.14.12.13 2,5-dichlorobenzoate + NADH + O2 involved in the metabolism of 2,5-dichlorobenzoate Pseudomonas sp. 4-chlorocatechol + NAD+ + Cl- + CO2
-
 ?
1.14.12.13 2,5-dichlorobenzoate + NADH + O2 involved in the metabolism of 2,5-dichlorobenzoate Pseudomonas sp. CPE2 4-chlorocatechol + NAD+ + Cl- + CO2
-
 ?
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2
-
 Pseudomonas sp. catechol + chloride + NAD+ + CO2
-
 ?
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2 involved in the metabolism of 2-chlorobenzoate Pseudomonas sp. catechol + chloride + NAD+ + CO2
-
 ?
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2
-
 Pseudomonas sp. CPE2 catechol + chloride + NAD+ + CO2
-
 ?
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2 involved in the metabolism of 2-chlorobenzoate Pseudomonas sp. CPE2 catechol + chloride + NAD+ + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.12.13 25
-
 assay at Pseudomonas sp.
1.14.12.13 30
-
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.12.13 5.9
-
-
 Pseudomonas sp.
1.14.12.13 6.5
-
 assay at Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.13 FAD
-
 Pseudomonas sp.
1.14.12.13 NADH exogenous Pseudomonas sp.