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Literature summary extracted from
- Degradation of 2-chlorobenzoate by Pseudomonas cepacia 2CBS (1989), Biol. Chem. Hoppe-Seyler, 370, 1173 - 1182.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.12.13 | additional information | mutants are made by treatment with N-methyl-N'-nitro-N-nitrosoguanidine, mutants are unable to grow on 2-chlorobenzoate and catechol | Burkholderia cepacia |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.12.13 | sucrose has stabilizing effect | Burkholderia cepacia |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.13 | Fe2+ | exogenous, required for activity | Burkholderia cepacia |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.12.13 | 2-chlorobenzoate + NADH + H+ + O2 | Burkholderia cepacia | first step of 2-chlorobenzoate degradation via meta-cleavage | catechol + chloride + NAD+ + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.12.13 | Burkholderia cepacia | - |
2CBS, isolated from water | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.12.13 | anion exchange | Burkholderia cepacia |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.12.13 | 2-chlorobenzoate + NADH + H+ + O2 = catechol + chloride + NAD+ + CO2 | multi-component dioxygenase system proposed: 1,2-hydroxylating, dehalogenating, decarboxylating | Burkholderia cepacia |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.13 | 0.0085 | - |
crude extract, 3.4 mg protein per ml in the assay | Burkholderia cepacia |
| 1.14.12.13 | 0.043 | - |
after anion exchange: fraction A + B + NADH + Fe2+ | Burkholderia cepacia |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.12.13 | 2-chlorobenzoate + NADH + H+ + O2 | the oxygen in the enzymatically catechol is derived from molecular oxygen, the formed byproduct 2,3-dihydroxybenzoate is suggested to be a dead end metabolite | Burkholderia cepacia | catechol + chloride + NAD+ + CO2 | - |
? | |
| 1.14.12.13 | 2-chlorobenzoate + NADH + H+ + O2 | first step of 2-chlorobenzoate degradation via meta-cleavage | Burkholderia cepacia | catechol + chloride + NAD+ + CO2 | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.13 | 25 | - |
assay at | Burkholderia cepacia |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.12.13 | 6.6 | - |
assay at | Burkholderia cepacia |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.12.13 | NADH | can not be replaced by NADPH | Burkholderia cepacia | |
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