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Literature summary extracted from

  • Mason, J.R.; Butler, C.S.; Cammack, R.; Shergill, J.K.
    Structural studies on the catalytic component of benzene dioxygenase from Pseudomonas putida (1997), Biochem. Soc. Trans., 25, 90-95.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.3 genes bedC1 and bedC2 encoding the terminal oxygenase alpha-subunit and beta-subunit, expression in Escherichia coli Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.3 H119C the mutant alpha-subunit of the terminal dioxygenase is unable to coordinate an EPR-detectable Rieske [2Fe-2S] cluster with the characteristic g factors Pseudomonas putida
1.14.12.3 H222M in the reconstitution assay with the reductase component, the ferredoxin component and the beta-subunit of terminal dioxygenase the mutant alpha-subunit is unable to reconstitute dioxygenase activity Pseudomonas putida
1.14.12.3 H228C in the reconstitution assay with the reductase component, the ferredoxin component and the beta-subunit of terminal dioxygenase the mutant alpha-subunit is unable to reconstitute dioxygenase activity Pseudomonas putida
1.14.12.3 H98C the mutant alpha-subunit of the terminal dioxygenase is unable to coordinate an EPR-detectable Rieske [2Fe-2S] cluster with the characteristic g factors, detection of a novel EPR spectrum, the intensity of the spectrum is approximately 8% from the wild-type Pseudomonas putida
1.14.12.3 Y118S the mutant alpha-subunit of the terminal dioxygenase shows an EPR spectrum of half the intensity of that of the wild-type. In the reconstitution assay with the reductase component, the ferredoxin component and the beta-subunit of terminal dioxygenase it shows significantly reduced activities Pseudomonas putida
1.14.12.3 Y221A in the reconstitution assay with the reductase component, the ferredoxin component and the beta-subunit of terminal dioxygenase the mutant alpha-subunit shows significantly reduced activity Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.3 55000
-
 55000 Da is the MW of the alpha-subunit of the terminal dioxygenase Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.3 benzene + NADH + O2 Pseudomonas putida reaction in benzene catabolism ?
-
 ?
1.14.12.3 benzene + NADH + O2 Pseudomonas putida ML2 reaction in benzene catabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.3 Pseudomonas putida
-
-
-
1.14.12.3 Pseudomonas putida ML2
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.3 benzene + NADH + O2
-
 Pseudomonas putida cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.3 benzene + NADH + O2
-
 Pseudomonas putida ML2 cis-cyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.3 benzene + NADH + O2 reaction in benzene catabolism Pseudomonas putida ?
-
 ?
1.14.12.3 benzene + NADH + O2 reaction in benzene catabolism Pseudomonas putida ML2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.3 More 55000 Da is the MW of the alpha-subunit of the terminal dioxygenase Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.3 Ferredoxin the benzene dioxygenase comprises three components: 1. flavoprotein, NADH:ferredoxin oxidoreductase, 2. an intermediate electron-transfer protein, or ferredoxin and 3. a terminal dioxygenase Pseudomonas putida