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Literature summary extracted from

  • Iwaki, H.; Hasegawa, Y.; Wang, S.; Kayser, M.M.; Lau, P.C.K.
    Cloning and characterization of a gene cluster involved in cyclopentanol metabolism in Comamonas sp. strain N.C.I.M.B. 9872 and biotransformations effected by Eschericha coli-expressed cyclopentanone 1,2-monooxygenase (2002), Appl. Environ. Microbiol., 68, 5671-5684.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.163
-
 Comamonas sp.
1.14.13.16 overexpression in Escherichia coli Comamonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.16 cyclopentanone + NADPH + O2 Comamonas sp.
-
 5-valerolactone + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.163 Comamonas sp.
-
-
-
1.14.13.16 Comamonas sp.
-
 reclassified, formerly named Pseudomonas sp. NCIB 9872
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.163 1,4-cyclohexandiol + NAD+ 20-30% of the activity with cyclopentanol Comamonas sp. 4-hydroxy-cyclohexanone + NADH
-
 ?
1.1.1.163 2-methylcyclohexanol + NAD+ 20-30% of the activity with cyclopentanol Comamonas sp. 2-methyl-cyclohexanone + NADH
-
 ?
1.1.1.163 cyclohexanol + NAD+ 60% of the activity with cyclopentanol Comamonas sp. cyclohexanone + NADH
-
 ?
1.1.1.163 cyclopentanol + NAD+
-
 Comamonas sp. cyclopentanone + NADH
-
 ?
1.14.13.16 cyclopentanone + NADPH + O2
-
 Comamonas sp. 5-valerolactone + NADP+ + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.163 NAD+
-
 Comamonas sp.
1.14.13.16 FAD
-
 Comamonas sp.
1.14.13.16 NADPH
-
 Comamonas sp.