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Literature summary extracted from

  • Makdessi, K.; Lechner, U.
    Purification and characterization of 2,4-dichlorophenol hydroxylase isolated from a bacterium of the alpha-2 subgroup of the Proteobacteria (1997), FEMS Microbiol. Lett., 157, 95-101.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.20 0.003
-
 2,4-Dichlorophenol
-
 Proteobacteria
1.14.13.20 0.024
-
 NADPH
-
 Proteobacteria
1.14.13.20 0.042
-
 NADH
-
 Proteobacteria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.20 64000
-
 alpha4, 4 * 64000, SDS-PAGE Proteobacteria
1.14.13.20 256000
-
 gel filtration, gradient PAGE Proteobacteria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.20 2,4-dichlorophenol + NADPH + O2 Proteobacteria
-
 3,5-dichlorocatechol + NADP+ + H2O
-
 ?
1.14.13.20 2,4-dichlorophenol + NADPH + O2 Proteobacteria S1
-
 3,5-dichlorocatechol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.20 Proteobacteria
-
 S1
-
1.14.13.20 Proteobacteria S1
-
 S1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.20
-
 Proteobacteria

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.20 1.8
-
 without FAD Proteobacteria
1.14.13.20 2.4
-
-
 Proteobacteria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.20 2,3-dichlorophenol + NADPH + O2
-
 Proteobacteria ?
-
 ?
1.14.13.20 2,3-dichlorophenol + NADPH + O2
-
 Proteobacteria S1 ?
-
 ?
1.14.13.20 2,4,5-trichlorophenol + NADPH + O2
-
 Proteobacteria ?
-
 ?
1.14.13.20 2,4,5-trichlorophenol + NADPH + O2
-
 Proteobacteria S1 ?
-
 ?
1.14.13.20 2,4,6-trichlorophenol + NADPH + O2
-
 Proteobacteria ?
-
 ?
1.14.13.20 2,4-dibromophenol + NADPH + O2
-
 Proteobacteria ?
-
 ?
1.14.13.20 2,4-dibromophenol + NADPH + O2
-
 Proteobacteria S1 ?
-
 ?
1.14.13.20 2,4-dichlorophenol + NADH + H+ + O2
-
 Proteobacteria 3,5-dichlorocatechol + NAD+ + H2O
-
 r
1.14.13.20 2,4-dichlorophenol + NADPH + O2
-
 Proteobacteria 3,5-dichlorocatechol + NADP+ + H2O
-
 ?
1.14.13.20 2,4-dichlorophenol + NADPH + O2
-
 Proteobacteria S1 3,5-dichlorocatechol + NADP+ + H2O
-
 ?
1.14.13.20 2,5-dichlorophenol + NADPH + O2
-
 Proteobacteria ?
-
 ?
1.14.13.20 2,6-dichlorophenol + NADPH + O2
-
 Proteobacteria ?
-
 ?
1.14.13.20 2-chlorophenol + NADPH + O2
-
 Proteobacteria ?
-
 ?
1.14.13.20 2-methylphenol + NADPH + O2
-
 Proteobacteria ?
-
 ?
1.14.13.20 3,4-dichlorophenol + NADPH + O2
-
 Proteobacteria ?
-
 ?
1.14.13.20 3,5-dichlorophenol + NADPH + O2
-
 Proteobacteria ?
-
 ?
1.14.13.20 3-chlorophenol + NADPH + O2
-
 Proteobacteria ?
-
 ?
1.14.13.20 4-bromophenol + NADPH + O2
-
 Proteobacteria ?
-
 ?
1.14.13.20 4-chloro-2-methylphenol + NADPH + O2
-
 Proteobacteria ?
-
 ?
1.14.13.20 4-chloro-2-methylphenol + NADPH + O2
-
 Proteobacteria S1 ?
-
 ?
1.14.13.20 4-chlorophenol + NADPH + O2
-
 Proteobacteria ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.20 tetramer alpha4, 4 * 64000, SDS-PAGE Proteobacteria

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.20 FAD 1 mol of enzyme contains: 4 mol FAD Proteobacteria
1.14.13.20 NADH
-
 Proteobacteria
1.14.13.20 NADPH
-
 Proteobacteria