BRENDA - Enzyme Database show

Purification and characterization of 2,4-dichlorophenol hydroxylase isolated from a bacterium of the alpha-2 subgroup of the Proteobacteria

Makdessi, K.; Lechner, U.; FEMS Microbiol. Lett. 157, 95-101 (1997)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.13.20
0.003
-
2,4-Dichlorophenol
-
Proteobacteria
1.14.13.20
0.024
-
NADPH
-
Proteobacteria
1.14.13.20
0.042
-
NADH
-
Proteobacteria
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.13.20
64000
-
alpha4, 4 * 64000, SDS-PAGE
Proteobacteria
1.14.13.20
256000
-
gel filtration, gradient PAGE
Proteobacteria
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.20
2,4-dichlorophenol + NADPH + O2
Proteobacteria
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + O2
Proteobacteria S1
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.20
Proteobacteria
-
S1
-
1.14.13.20
Proteobacteria S1
-
S1
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.13.20
-
Proteobacteria
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.20
1.8
-
without FAD
Proteobacteria
1.14.13.20
2.4
-
-
Proteobacteria
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.20
2,3-dichlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
2,3-dichlorophenol + NADPH + O2
-
439016
Proteobacteria S1
?
-
-
-
?
1.14.13.20
2,4,5-trichlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
2,4,5-trichlorophenol + NADPH + O2
-
439016
Proteobacteria S1
?
-
-
-
?
1.14.13.20
2,4,6-trichlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
2,4-dibromophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
2,4-dibromophenol + NADPH + O2
-
439016
Proteobacteria S1
?
-
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + O2
-
439016
Proteobacteria
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + O2
-
439016
Proteobacteria S1
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2,5-dichlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
2,6-dichlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
2-chlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
2-methylphenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
3,4-dichlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
3,5-dichlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
3-chlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
4-bromophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
4-chloro-2-methylphenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
4-chloro-2-methylphenol + NADPH + O2
-
439016
Proteobacteria S1
?
-
-
-
?
1.14.13.20
4-chlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.13.20
tetramer
alpha4, 4 * 64000, SDS-PAGE
Proteobacteria
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.20
FAD
1 mol of enzyme contains: 4 mol FAD
Proteobacteria
1.14.13.20
NADH
-
Proteobacteria
1.14.13.20
NADPH
-
Proteobacteria
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.20
FAD
1 mol of enzyme contains: 4 mol FAD
Proteobacteria
1.14.13.20
NADH
-
Proteobacteria
1.14.13.20
NADPH
-
Proteobacteria
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.13.20
0.003
-
2,4-Dichlorophenol
-
Proteobacteria
1.14.13.20
0.024
-
NADPH
-
Proteobacteria
1.14.13.20
0.042
-
NADH
-
Proteobacteria
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.13.20
64000
-
alpha4, 4 * 64000, SDS-PAGE
Proteobacteria
1.14.13.20
256000
-
gel filtration, gradient PAGE
Proteobacteria
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.20
2,4-dichlorophenol + NADPH + O2
Proteobacteria
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + O2
Proteobacteria S1
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.20
-
Proteobacteria
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.20
1.8
-
without FAD
Proteobacteria
1.14.13.20
2.4
-
-
Proteobacteria
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.20
2,3-dichlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
2,3-dichlorophenol + NADPH + O2
-
439016
Proteobacteria S1
?
-
-
-
?
1.14.13.20
2,4,5-trichlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
2,4,5-trichlorophenol + NADPH + O2
-
439016
Proteobacteria S1
?
-
-
-
?
1.14.13.20
2,4,6-trichlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
2,4-dibromophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
2,4-dibromophenol + NADPH + O2
-
439016
Proteobacteria S1
?
-
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + O2
-
439016
Proteobacteria
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + O2
-
439016
Proteobacteria S1
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2,5-dichlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
2,6-dichlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
2-chlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
2-methylphenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
3,4-dichlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
3,5-dichlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
3-chlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
4-bromophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
4-chloro-2-methylphenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
1.14.13.20
4-chloro-2-methylphenol + NADPH + O2
-
439016
Proteobacteria S1
?
-
-
-
?
1.14.13.20
4-chlorophenol + NADPH + O2
-
439016
Proteobacteria
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.13.20
tetramer
alpha4, 4 * 64000, SDS-PAGE
Proteobacteria