BRENDA - Enzyme Database show

The 2,4-dichlorophenol hydroxylase of Alcaligenes eutrophus JMP134 is a homotetramer

Farhana, L.; New, P.B.; Can. J. Microbiol. 43, 202-205 (1997)

Data extracted from this reference:

Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.13.20
66000
-
alpha4, 4 * 66000, SDS-PAGE
Cupriavidus necator
1.14.13.20
245000
-
gel filtration
Cupriavidus necator
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.20
2,4-dichlorophenol + NADPH + O2
Cupriavidus necator
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + O2
Cupriavidus necator JMP 134-1
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.20
Cupriavidus necator
-
basonym Alcaligenes eutrophus; JMP134
-
1.14.13.20
Cupriavidus necator JMP 134-1
-
JMP134
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.13.20
-
Cupriavidus necator
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.20
1.14
-
-
Cupriavidus necator
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.20
2,4-dichlorophenol + NADPH + O2
-
439015
Cupriavidus necator
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + O2
-
439015
Cupriavidus necator JMP 134-1
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.13.20
tetramer
alpha4, 4 * 66000, SDS-PAGE
Cupriavidus necator
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.13.20
66000
-
alpha4, 4 * 66000, SDS-PAGE
Cupriavidus necator
1.14.13.20
245000
-
gel filtration
Cupriavidus necator
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.20
2,4-dichlorophenol + NADPH + O2
Cupriavidus necator
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + O2
Cupriavidus necator JMP 134-1
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.20
-
Cupriavidus necator
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.20
1.14
-
-
Cupriavidus necator
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.20
2,4-dichlorophenol + NADPH + O2
-
439015
Cupriavidus necator
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2,4-dichlorophenol + NADPH + O2
-
439015
Cupriavidus necator JMP 134-1
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.13.20
tetramer
alpha4, 4 * 66000, SDS-PAGE
Cupriavidus necator