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Literature summary extracted from
- Affinity purification and characterization of 2,4-dichlorophenol hydroxylase from Pseudomonas cepacia [published erratum appears in Arch Biochem Biophys 1992 Jul;296(1):354] (1991), Arch. Biochem. Biophys., 288, 169-176.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.20 | 2,4,5-Trichlorophenol | - |
Burkholderia cepacia |  |
| 1.14.13.20 | 2,4,6-Trichlorophenol | - |
Burkholderia cepacia | |
| 1.14.13.20 | guanidine hydrochloride | - |
Burkholderia cepacia | |
| 1.14.13.20 | Hg2+ | - |
Burkholderia cepacia | |
| 1.14.13.20 | p-chloromercuribenzoate | - |
Burkholderia cepacia | |
| 1.14.13.20 | SDS | - |
Burkholderia cepacia | |
| 1.14.13.20 | Urea | - |
Burkholderia cepacia | |
| 1.14.13.20 | Zn2+ | - |
Burkholderia cepacia | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.20 | 0.014 | - |
2,4-Dichlorophenol | - |
Burkholderia cepacia | |
| 1.14.13.20 | 0.1 | - |
NADPH | - |
Burkholderia cepacia | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.20 | 69000 | - |
alpha4, 4 * 69000, SDS-PAGE | Burkholderia cepacia |
| 1.14.13.20 | 275000 | - |
gel filtration and ultracentrifugation | Burkholderia cepacia |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.20 | 2,4-dichlorophenol + NADPH + O2 | Burkholderia cepacia | - |
3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.20 | Burkholderia cepacia | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.20 | single step affinity chromatography | Burkholderia cepacia |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.20 | 1.9 | - |
- |
Burkholderia cepacia |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.20 | 2,4-dichlorophenol + NADPH + O2 | - |
Burkholderia cepacia | 3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.20 | tetramer | alpha4, 4 * 69000, SDS-PAGE | Burkholderia cepacia |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.20 | 37 | - |
- |
Burkholderia cepacia |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.20 | 8.5 | - |
- |
Burkholderia cepacia |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.20 | FAD | flavoprotein | Burkholderia cepacia | |
| 1.14.13.20 | FAD | 1 mol of enzyme contains: 4 mol FAD | Burkholderia cepacia | |
| 1.14.13.20 | FAD | requires exogenous addition of FAD | Burkholderia cepacia | |
| 1.14.13.20 | NADH | NADPH preferred to NADH | Burkholderia cepacia | |
| 1.14.13.20 | NADPH | - |
Burkholderia cepacia | |
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