BRENDA - Enzyme Database show

The purification and properties of 2,4-dichlorophenol hydroxylase from a strain of Acinetobacter species

Beadle, C.A.; Smith, A.R.W.; Eur. J. Biochem. 123, 323-332 (1982)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.14.13.20
2,4-dichlorophenol, 0.1 mM, stabilizes during purification
Acinetobacter sp.
1.14.13.20
dithiothreitol, 1 mM, stabilizes during purification
Acinetobacter sp.
1.14.13.20
EDTA, 0.1 mM, stabilizes during purification
Acinetobacter sp.
1.14.13.20
FAD, 0.01 mM, stabilizes during purification
Acinetobacter sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.13.20
2,4-Dichlorophenol
above 1 mM
Acinetobacter sp.
1.14.13.20
AgNO3
-
Acinetobacter sp.
1.14.13.20
CuSO4
-
Acinetobacter sp.
1.14.13.20
diethyldithiocarbamate
-
Acinetobacter sp.
1.14.13.20
dithiothreitol
slight
Acinetobacter sp.
1.14.13.20
FeCl3
-
Acinetobacter sp.
1.14.13.20
FeSO4
-
Acinetobacter sp.
1.14.13.20
Hg2+
HgCl2
Acinetobacter sp.
1.14.13.20
KCN
-
Acinetobacter sp.
1.14.13.20
p-chloromercuribenzoate
-
Acinetobacter sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.13.20
0.004
-
2,4-Dichlorophenol
cosubstrate NADPH
Acinetobacter sp.
1.14.13.20
0.057
-
O2
-
Acinetobacter sp.
1.14.13.20
0.083
-
NADPH
-
Acinetobacter sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.13.20
63000
-
alpha4, 4 * 63000, SDS-PAGE
Acinetobacter sp.
1.14.13.20
240000
-
gel filtration
Acinetobacter sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.20
2,4-dichlorophenol + NADPH + O2
Acinetobacter sp.
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.20
Acinetobacter sp.
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.13.20
-
Acinetobacter sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.20
1.46
-
-
Acinetobacter sp.
Storage Stability
EC Number
Storage Stability
Organism
1.14.13.20
-20°C, 3 months stable
Acinetobacter sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.20
2,4-dichlorophenol + NADPH + O2
-
439011
Acinetobacter sp.
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2-chlorophenol + NADPH + O2
incompletely metabolized
439011
Acinetobacter sp.
?
-
-
-
?
1.14.13.20
2-cresol + NADPH + O2
incompletely metabolized
439011
Acinetobacter sp.
?
-
-
-
?
1.14.13.20
4-bromophenol + NADPH + O2
incompletely metabolized
439011
Acinetobacter sp.
?
-
-
-
?
1.14.13.20
4-chloro-2-methylphenol + NADPH + O2
-
439011
Acinetobacter sp.
?
-
-
-
?
1.14.13.20
4-chlorophenol + NADPH + O2
-
439011
Acinetobacter sp.
?
-
-
-
?
1.14.13.20
additional information
pseudosubstrates evoke oxidation of NAD(P)H and oxygen consumption without themselves undergoing hydroxylation, the product is hydrogen peroxide: 2,5-dichlorophenol, 2,6-dichlorophenol, 3,4-dichlorophenol, 2, 4,5-trichlorophenol
439011
Acinetobacter sp.
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.14.13.20
tetramer
alpha4, 4 * 63000, SDS-PAGE
Acinetobacter sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.13.20
29
-
-
Acinetobacter sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.13.20
6.83
-
2,4-Dichlorophenol
-
Acinetobacter sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.13.20
7.6
-
-
Acinetobacter sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.20
FAD
1 mol of enzyme contains: 1.9 mol FAD; FAD cannot be replaced by riboflavin or FMN; flavoprotein
Acinetobacter sp.
1.14.13.20
NADH
5% of the activity with NADPH
Acinetobacter sp.
1.14.13.20
NADPH
-
Acinetobacter sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.20
FAD
1 mol of enzyme contains: 1.9 mol FAD; FAD cannot be replaced by riboflavin or FMN; flavoprotein
Acinetobacter sp.
1.14.13.20
NADH
5% of the activity with NADPH
Acinetobacter sp.
1.14.13.20
NADPH
-
Acinetobacter sp.
General Stability (protein specific)
EC Number
General Stability
Organism
1.14.13.20
2,4-dichlorophenol, 0.1 mM, stabilizes during purification
Acinetobacter sp.
1.14.13.20
dithiothreitol, 1 mM, stabilizes during purification
Acinetobacter sp.
1.14.13.20
EDTA, 0.1 mM, stabilizes during purification
Acinetobacter sp.
1.14.13.20
FAD, 0.01 mM, stabilizes during purification
Acinetobacter sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.13.20
2,4-Dichlorophenol
above 1 mM
Acinetobacter sp.
1.14.13.20
AgNO3
-
Acinetobacter sp.
1.14.13.20
CuSO4
-
Acinetobacter sp.
1.14.13.20
diethyldithiocarbamate
-
Acinetobacter sp.
1.14.13.20
dithiothreitol
slight
Acinetobacter sp.
1.14.13.20
FeCl3
-
Acinetobacter sp.
1.14.13.20
FeSO4
-
Acinetobacter sp.
1.14.13.20
Hg2+
HgCl2
Acinetobacter sp.
1.14.13.20
KCN
-
Acinetobacter sp.
1.14.13.20
p-chloromercuribenzoate
-
Acinetobacter sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.13.20
0.004
-
2,4-Dichlorophenol
cosubstrate NADPH
Acinetobacter sp.
1.14.13.20
0.057
-
O2
-
Acinetobacter sp.
1.14.13.20
0.083
-
NADPH
-
Acinetobacter sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.13.20
63000
-
alpha4, 4 * 63000, SDS-PAGE
Acinetobacter sp.
1.14.13.20
240000
-
gel filtration
Acinetobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.20
2,4-dichlorophenol + NADPH + O2
Acinetobacter sp.
-
3,5-dichlorocatechol + NADP+ + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.20
-
Acinetobacter sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.20
1.46
-
-
Acinetobacter sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.13.20
-20°C, 3 months stable
Acinetobacter sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.20
2,4-dichlorophenol + NADPH + O2
-
439011
Acinetobacter sp.
3,5-dichlorocatechol + NADP+ + H2O
-
-
-
?
1.14.13.20
2-chlorophenol + NADPH + O2
incompletely metabolized
439011
Acinetobacter sp.
?
-
-
-
?
1.14.13.20
2-cresol + NADPH + O2
incompletely metabolized
439011
Acinetobacter sp.
?
-
-
-
?
1.14.13.20
4-bromophenol + NADPH + O2
incompletely metabolized
439011
Acinetobacter sp.
?
-
-
-
?
1.14.13.20
4-chloro-2-methylphenol + NADPH + O2
-
439011
Acinetobacter sp.
?
-
-
-
?
1.14.13.20
4-chlorophenol + NADPH + O2
-
439011
Acinetobacter sp.
?
-
-
-
?
1.14.13.20
additional information
pseudosubstrates evoke oxidation of NAD(P)H and oxygen consumption without themselves undergoing hydroxylation, the product is hydrogen peroxide: 2,5-dichlorophenol, 2,6-dichlorophenol, 3,4-dichlorophenol, 2, 4,5-trichlorophenol
439011
Acinetobacter sp.
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.13.20
tetramer
alpha4, 4 * 63000, SDS-PAGE
Acinetobacter sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.13.20
29
-
-
Acinetobacter sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.13.20
6.83
-
2,4-Dichlorophenol
-
Acinetobacter sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.13.20
7.6
-
-
Acinetobacter sp.