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Literature summary extracted from

  • Larson, R.L.; Bussard, J.B.
    Microsomal flavonoid 3'-monooxygenase from maize seedlings (1986), Plant Physiol., 80, 483-486.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.14.14.82 sucrose or glycerol plus dithiothreitol stabilize during purification and storage at -70°C Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.82 alpha,alpha'-dipyridyl partial Zea mays
1.14.14.82 CO inhibition reduced by irradiation with 450 nm light during incubation Zea mays
1.14.14.82 cytochrome c
-
 Zea mays
1.14.14.82 FeCl3
-
 Zea mays
1.14.14.82 N-ethylmaleimide
-
 Zea mays
1.14.14.82 NADP+
-
 Zea mays
1.14.14.82 NaN3
-
 Zea mays
1.14.14.82 p-chloromercuribenzoate partial Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.82 microsome
-
 Zea mays
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.82 Zea mays
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.82 seedling
-
 Zea mays
-

Storage Stability

EC Number Storage Stability Organism
1.14.14.82 -70°C, sucrose or glycerol plus dithiothreitol stabilize during purification and storage Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.82 apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Zea mays luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 no activity Zea mays dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Zea mays quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Zea mays eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.82 30
-
-
 Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.82 100
-
 10 min, activity destroyed Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.82 8.5
-
-
 Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.14.82 additional information
-
 sharp drop in activity on either side of the optimum of pH 8.5 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.82 cytochrome P450 0.21 nmol per mg of protein Zea mays