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Literature summary extracted from

  • Stich, K.; Forkmann, G.
    Studies on columnidin biosynthesis with flower extracts from Columnea hybrida (1988), Z. Naturforsch. C, 43, 311-314.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.82 0.00715
-
 kaempferol
-
 Columnea hybrida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.234 soluble
-
 Columnea hybrida
-
-
1.14.14.82 microsome
-
 Columnea hybrida
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.234 (2S)-flavanone + NADPH Columnea hybrida involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol (2S)-flavan-4-ol + NADP+
-
 ?
1.1.1.234 (2S)-flavanone + NADPH Columnea hybrida Heklua involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol (2S)-flavan-4-ol + NADP+
-
 ?
1.14.14.82 apigenin + [reduced NADPH-hemoprotein reductase] + O2 Columnea hybrida
-
 luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 apigenin + [reduced NADPH-hemoprotein reductase] + O2 Columnea hybrida Heklua
-
 luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.234 Columnea hybrida
-
 strain "Heklua"
-
1.1.1.234 Columnea hybrida Heklua
-
 strain "Heklua"
-
1.14.14.82 Columnea hybrida
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.234 flower
-
 Columnea hybrida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.234 0.0009
-
-
 Columnea hybrida
1.14.14.82 additional information
-
-
 Columnea hybrida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.234 (2S)-eriodictyol + NADPH
-
 Columnea hybrida luteoforol + NADP+
-
 ?
1.1.1.234 (2S)-eriodictyol + NADPH
-
 Columnea hybrida Heklua luteoforol + NADP+
-
 ?
1.1.1.234 (2S)-flavanone + NADPH involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol Columnea hybrida (2S)-flavan-4-ol + NADP+
-
 ?
1.1.1.234 (2S)-flavanone + NADPH involved in the biosynthesis of 3-deoxyanthocyanidines such as apigeninidin or luteolinidin, from (2S)-flavanones naringenin or eriodictyol Columnea hybrida Heklua (2S)-flavan-4-ol + NADP+
-
 ?
1.1.1.234 (2S)-naringenin + NADPH + H+
-
 Columnea hybrida apiforol + NADP+
-
 ?
1.1.1.234 (2S)-naringenin + NADPH + H+
-
 Columnea hybrida Heklua apiforol + NADP+
-
 ?
1.14.14.82 apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Columnea hybrida luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Columnea hybrida Heklua luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Columnea hybrida quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.234 5.5
-
-
 Columnea hybrida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.234 NADPH required Columnea hybrida