Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hasegawa, Y.; Nakai, Y.; Tokuyama, T.; Iwaki, H.
    Purification and characterization of cyclohexanone 1,2-monooxygenase from Exophiala jeanselmei strain KUFI-6N (2000), Biosci. Biotechnol. Biochem., 64, 2696-2698.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.22 5,5'-dithiobis(2-nitrobenzoate) completely Exophiala jeanselmei
1.14.13.22 Cu2+ completely Exophiala jeanselmei
1.14.13.22 Quinacrine
-
 Exophiala jeanselmei
1.14.13.22 Quinine
-
 Exophiala jeanselmei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.22 0.00048
-
 cyclohexanone
-
 Exophiala jeanselmei
1.14.13.22 0.0142
-
 NADPH
-
 Exophiala jeanselmei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.22 74000
-
 gel filtration Exophiala jeanselmei

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.22 Exophiala jeanselmei
-
-
-
1.14.13.22 Exophiala jeanselmei
-
 inducible by cyclohexanone
-
1.14.13.22 Exophiala jeanselmei KUFI-6N
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.22
-
 Exophiala jeanselmei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.22 678
-
 purified enzyme Exophiala jeanselmei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.22 cyclobutanone + NADPH + O2
-
 Exophiala jeanselmei 1-oxa-2-oxo-cyclopentane + NADP+ + H2O
-
 ?
1.14.13.22 cyclobutanone + NADPH + O2
-
 Exophiala jeanselmei KUFI-6N 1-oxa-2-oxo-cyclopentane + NADP+ + H2O
-
 ?
1.14.13.22 cyclodecanone + NADPH + O2 low activity Exophiala jeanselmei 1-oxa-2-oxocycloendecane + NADP+ + H2O
-
 ?
1.14.13.22 cycloheptanone + NADPH + O2
-
 Exophiala jeanselmei 1-oxa-2-oxo-cyclooctane + NADP+ + H2O
-
 ?
1.14.13.22 cycloheptanone + NADPH + O2
-
 Exophiala jeanselmei KUFI-6N 1-oxa-2-oxo-cyclooctane + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexanone + NADPH + O2
-
 Exophiala jeanselmei 6-hexanolide + NADP+ + H2O product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane ?
1.14.13.22 cyclohexanone + NADPH + O2
-
 Exophiala jeanselmei KUFI-6N 6-hexanolide + NADP+ + H2O product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane ?
1.14.13.22 cyclooctanone + NADPH + O2
-
 Exophiala jeanselmei 1-oxa-2-oxo-cyclononane + NADP+ + H2O
-
 ?
1.14.13.22 cyclooctanone + NADPH + O2
-
 Exophiala jeanselmei KUFI-6N 1-oxa-2-oxo-cyclononane + NADP+ + H2O
-
 ?
1.14.13.22 cyclopentanone + NADPH + O2
-
 Exophiala jeanselmei 1-oxa-2-oxo-cyclohexane + NADP+ + H2O
-
 ?
1.14.13.22 cyclopentanone + NADPH + O2
-
 Exophiala jeanselmei KUFI-6N 1-oxa-2-oxo-cyclohexane + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.22 monomer 1 * 74000, SDS-PAGE Exophiala jeanselmei

Synonyms

EC Number Synonyms Comment Organism
1.14.13.22 CHMO
-
 Exophiala jeanselmei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.22 30
-
 assay at Exophiala jeanselmei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.22 8
-
-
 Exophiala jeanselmei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.22 5.5 10.5 no activity below pH 5.5 and above pH 10.5 at 30°C Exophiala jeanselmei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.13.22 7 8 stable Exophiala jeanselmei

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.22 FAD binding motif, comparison of N-terminal amino acid sequences of several species Exophiala jeanselmei
1.14.13.22 NADPH
-
 Exophiala jeanselmei