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Literature summary extracted from

  • Cheesman, M.J.; Kneller, M.B.; Kelly, E.J.; Thompson, S.J.; Yeung, C.K.; Eaton, D.L.; Rettie, A.E.
    purification and characterization of hexahistidine-tagged cyclohexanone monooxygenase expressed in Saccharomyces cerevisiae and Escherichia coli (2001), Protein Expr. Purif., 21, 81-86.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.22 expression of His-tagged enzyme in Escherichia coli and Saccharomyces cerevisiae Acinetobacter sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.22 additional information His-tagged recombinant enzyme expressed in Escherichia coli is, despite the tag, similar to the wild-type enzyme, whereas the recombinant His-tagged enzyme expressed in yeast is posttranslationally modified, Escherichia coli is the preferred expression system Acinetobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.22 60800
-
 wild-type, electrospray mass spectrometry Acinetobacter sp.
1.14.13.22 61620
-
 recombinant His-tagged enzyme expressed in E. coli Acinetobacter sp.
1.14.13.22 61670
-
 recombinant His-tagged enzyme expressed in yeast Acinetobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.22 Acinetobacter sp. Q9R2F5 NCIB 9871
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.22 native enzyme and recombinant His-tagged enzyme from Escherichia coli and Saccharomyces cerevisiae Acinetobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.22 14
-
 purified recombinant His-tagged enzyme expressed in yeast Acinetobacter sp.
1.14.13.22 15
-
 purified native enzyme Acinetobacter sp.
1.14.13.22 19.9
-
 purified recombinant His-tagged enzyme expressed in E. coli Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.22 cyclohexanone + NADPH + O2
-
 Acinetobacter sp. 6-hexanolide + NADP+ + H2O product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.22 CMO
-
 Acinetobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.22 FAD
-
 Acinetobacter sp.
1.14.13.22 NADPH
-
 Acinetobacter sp.