Literature summary extracted from
Sheng, D.; Ballou, D.P.; Massey, V.
Mechanistic studies of cyclohexanone monooxygenase: chemical properties of intermediates involved in catalysis (2001), Biochemistry, 40, 11156-11167.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.13.22 |
expression in Escherichia coli |
Acinetobacter sp. |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.14.13.22 |
NADP+ |
competitive against NADPH |
Acinetobacter sp. |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.13.22 |
Acinetobacter sp. |
- |
NCIB 9871 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.13.22 |
recombinant from Escherichia coli |
Acinetobacter sp. |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
1.14.13.22 |
cyclohexanone + NADPH + H+ + O2 = hexano-6-lactone + NADP+ + H2O |
reaction mechanism |
Acinetobacter sp. |
|
1.14.13.22 |
cyclohexanone + NADPH + H+ + O2 = hexano-6-lactone + NADP+ + H2O |
kinetic studies |
Acinetobacter sp. |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.13.22 |
cyclohexanone + NADPH + O2 |
activity limited to cyclic ketones |
Acinetobacter sp. |
6-hexanolide + NADP+ + H2O |
product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.13.22 |
CHMO |
- |
Acinetobacter sp. |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.14.13.22 |
25 |
- |
assay at |
Acinetobacter sp. |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.13.22 |
FAD |
- |
Acinetobacter sp. |
|
1.14.13.22 |
NADPH |
- |
Acinetobacter sp. |
|
Ki Value [mM]
EC Number |
Ki Value [mM] |
Ki Value maximum [mM] |
Inhibitor |
Comment |
Organism |
Structure |
---|
1.14.13.22 |
0.038 |
- |
NADP+ |
pH 9.0, 25°C |
Acinetobacter sp. |
|