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Literature summary extracted from

  • Ougham, H.J.; Trudgill, P.W.
    Metabolism of cyclohexaneacetic acid and cyclohexanebutyric acid by Arthrobacter sp. strain CA1 (1982), J. Bacteriol., 150, 1172-1182.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
4.1.3.35 increasingly unstable upon further purification Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.22 cyclohexanone + NADPH + O2 Arthrobacter sp. pathway 1-oxa-2-oxocycloheptane + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexanone + NADPH + O2 Arthrobacter sp. CA1 pathway 1-oxa-2-oxocycloheptane + NADP+ + H2O
-
 ?
4.1.3.35 (1-hydroxycyclohexan-1-yl)acetyl-CoA Arthrobacter sp.
-
 acetyl-CoA + cyclohexanone
-
 ?
4.1.3.35 (1-hydroxycyclohexan-1-yl)acetyl-CoA Arthrobacter sp. CA1
-
 acetyl-CoA + cyclohexanone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.22 Arthrobacter sp.
-
 low activity
-
1.14.13.22 Arthrobacter sp. CA1
-
 low activity
-
4.1.3.35 Arthrobacter sp.
-
-
-
4.1.3.35 Arthrobacter sp. CA1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.35 partial Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.22 cyclohexanone + NADPH + O2
-
 Arthrobacter sp. 6-hexanolide + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexanone + NADPH + O2
-
 Arthrobacter sp. CA1 6-hexanolide + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexanone + NADPH + O2 pathway Arthrobacter sp. 1-oxa-2-oxocycloheptane + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexanone + NADPH + O2 pathway Arthrobacter sp. CA1 1-oxa-2-oxocycloheptane + NADP+ + H2O
-
 ?
4.1.3.35 (1-hydroxycyclohexan-1-yl)acetyl-CoA
-
 Arthrobacter sp. acetyl-CoA + cyclohexanone
-
 ?
4.1.3.35 (1-hydroxycyclohexan-1-yl)acetyl-CoA
-
 Arthrobacter sp. CA1 acetyl-CoA + cyclohexanone
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.35 30
-
 assay at Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.35 8
-
 assay at Arthrobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.22 NADPH
-
 Arthrobacter sp.