EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.22 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Xanthobacter sp. | |
1.14.13.22 | arsenate | - |
Xanthobacter sp. | |
1.14.13.22 | CuCl2 | - |
Xanthobacter sp. | |
1.14.13.22 | FeCl3 | - |
Xanthobacter sp. | |
1.14.13.22 | p-hydroxymercuribenzoate | - |
Xanthobacter sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.22 | 0.0005 | - |
cyclohexanone | below | Xanthobacter sp. | |
1.14.13.22 | 0.0125 | - |
NADPH | - |
Xanthobacter sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.13.22 | cytoplasm | - |
Xanthobacter sp. | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.22 | 50000 | - |
gel filtration | Xanthobacter sp. |
1.14.13.22 | 51000 | - |
1 * 51000, SDS-PAGE | Xanthobacter sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.22 | Xanthobacter sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.22 | - |
Xanthobacter sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.22 | 2.1 | - |
- |
Xanthobacter sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.13.22 | -20°C, stable | Xanthobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.22 | 2-hydroxycyclohexanone + NADPH + O2 | - |
Xanthobacter sp. | 1-oxa-2-oxo-3-hydroxycycloheptane + NADP+ + H2O | - |
? | |
1.14.13.22 | 2-methylcyclohexanone + NADPH + O2 | - |
Xanthobacter sp. | 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O | - |
? | |
1.14.13.22 | cyclobutanone + NADPH + O2 | - |
Xanthobacter sp. | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
1.14.13.22 | cycloheptanone + NADPH + O2 | - |
Xanthobacter sp. | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
1.14.13.22 | cyclohexan-1,2-dione + NADPH + O2 | - |
Xanthobacter sp. | 1-oxa-2,3-dioxo-cycloheptane + NADP+ + H2O | - |
? | |
1.14.13.22 | cyclohexan-1,4-dione + NADPH + O2 | - |
Xanthobacter sp. | 1-oxa-2,5-dioxo-cycloheptane + NADP+ + H2O | - |
? | |
1.14.13.22 | cyclohexanone + NADPH + O2 | - |
Xanthobacter sp. | 6-hexanolide + NADP+ + H2O | - |
? | |
1.14.13.22 | cyclooctanone + NADPH + O2 | low activity | Xanthobacter sp. | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
1.14.13.22 | cyclopentanone + NADPH + O2 | - |
Xanthobacter sp. | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
1.14.13.22 | additional information | no activity towards straight-chain alkanones | Xanthobacter sp. | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.22 | monomer | 1 * 51000, SDS-PAGE | Xanthobacter sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.22 | 30 | - |
assay at | Xanthobacter sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.22 | 8.8 | - |
- |
Xanthobacter sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.22 | 6 | 11 | at pH 6.0 and 11.0: about 10% of activity maximum | Xanthobacter sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.22 | FAD | - |
Xanthobacter sp. | |
1.14.13.22 | FMN | involved in catalytic mechanism | Xanthobacter sp. | |
1.14.13.22 | FMN | 1 FMN molecule per molecule of protein | Xanthobacter sp. | |
1.14.13.22 | NADPH | electron donor | Xanthobacter sp. |