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Literature summary extracted from

  • Suarez, M.; Ferrer, E.; Garrido-Pertierra, A.; Martin, M.
    Purification and characterization of the 3-hydroxybenzoate-6-hydroxylase from Klebsiella pneumoniae (1995), FEMS Microbiol. Lett., 126, 283-290.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.13.24 complete inactivation at room temperature without glycerol after 18 h Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.24 Cu2+ inhibition at 0.02 mM, partially reversed by 2-mercaptoethanol Klebsiella pneumoniae
1.14.13.24 Fe2+ inhibition at 0.02 mM, partially reversed by 2-mercaptoethanol Klebsiella pneumoniae
1.14.13.24 Hg2+ inhibition at 0.02 mM, partially reversed by 2-mercaptoethanol Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.24 0.07
-
 NADPH
-
 Klebsiella pneumoniae
1.14.13.24 0.07
-
 NADH
-
 Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.24 41500
-
 gel filtration Klebsiella pneumoniae
1.14.13.24 42000
-
 1 * 42000, SDS-PAGE Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.24 Klebsiella pneumoniae
-
-
-
1.14.13.24 Klebsiella pneumoniae M5a1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.24 homogeneity Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.24 8.42
-
-
 Klebsiella pneumoniae

Storage Stability

EC Number Storage Stability Organism
1.14.13.24 4°C, optimal storage in 50 mM phosphate or Tris buffer pH 8 with 10% glycerol Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.24 3-hydroxybenzoate + NADH + O2
-
 Klebsiella pneumoniae 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?
1.14.13.24 3-hydroxybenzoate + NADH + O2
-
 Klebsiella pneumoniae M5a1 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?
1.14.13.24 3-hydroxybenzoate + NADPH + H+ + O2
-
 Klebsiella pneumoniae 2,5-dihydroxybenzoate + NADP+ + H2O
-
 r
1.14.13.24 3-hydroxybenzoate + NADPH + H+ + O2
-
 Klebsiella pneumoniae M5a1 2,5-dihydroxybenzoate + NADP+ + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
1.14.13.24 monomer 1 * 42000, SDS-PAGE Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.24 25 30
-
 Klebsiella pneumoniae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.13.24 15 40 no activity below 15°C and above 40°C Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.24 8
-
-
 Klebsiella pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.24 6.5 11 no activity below pH 6.5 and above pH 11 Klebsiella pneumoniae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.13.24 7 8.5 in phosphate and Tris-HCl buffer Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.24 FAD
-
 Klebsiella pneumoniae
1.14.13.24 NADPH NADH and NADPH utilized with similar efficiencies Klebsiella pneumoniae