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Literature summary extracted from

  • Rajasekharan, S.; Rajasekharan, R.; Vaidyanathan, C.S.
    Substrate-mediated purification and characterization of a 3-hydroxybenzoic acid-6-hydroxylase from Micrococcus (1990), Arch. Biochem. Biophys., 278, 21-25.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.24 Cibacron blue 50% inhibition at 0.04 mM Micrococcus sp.
1.14.13.24 Cu2+ 85% inhibition at 0.01 mM, inhibition can be reversed by thiol reagents Micrococcus sp.
1.14.13.24 Hg2+ 85% inhibition at 0.01 mM, inhibition can be reversed by thiol reagents Micrococcus sp.
1.14.13.24 p-chloromercuribenzoate at low concentrations Micrococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.24 0.063
-
 3-hydroxybenzoate cosubstrate NADH Micrococcus sp.
1.14.13.24 0.095
-
 NADH
-
 Micrococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.24 additional information no requirement for metal ions Micrococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.24 67000
-
 SDS-PAGE Micrococcus sp.
1.14.13.24 70000
-
 gel filtration Micrococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.24 3-hydroxybenzoate + NADH + O2 Micrococcus sp. key role in metabolism of aromatic compounds 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.24 Micrococcus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.24 single step purification using substrate-mediated interaction of the enzyme with blue-Sepharose, homogeneity Micrococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.24 21.4
-
-
 Micrococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.24 3-hydroxybenzoate + NADH + O2 strictly specific for 3-hydroxybenzoate Micrococcus sp. 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?
1.14.13.24 3-hydroxybenzoate + NADH + O2 key role in metabolism of aromatic compounds Micrococcus sp. 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?
1.14.13.24 additional information not: salicylate Micrococcus sp. ?
-
 ?
1.14.13.24 additional information acts also on a number of analogs of 3-hydroxybenzoate substituted in the 2, 3, 5 and 6 positions Micrococcus sp. ?
-
 ?
1.14.13.24 additional information not: p-hydroxybenzoate Micrococcus sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.24 monomer 1 * 67000, SDS-PAGE Micrococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.24 8
-
-
 Micrococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.24 FAD flavoprotein Micrococcus sp.
1.14.13.24 FAD 1 mol FAD per mol enzyme, exogenous addition of FAD required to regenerate oxidized FAD during catalysis Micrococcus sp.
1.14.13.24 NADH
-
 Micrococcus sp.
1.14.13.24 NADPH strict preference for NADH over NADPH Micrococcus sp.