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Literature summary extracted from
- Pseudomonas cepacia 3-hydroxybenzoate 6-hydroxylase: stereochemistry, isotope effects, and kinetic mechanism (1987), Biochemistry, 26, 1105-1110.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.24 | 0.046 | - |
NADH | - |
Burkholderia cepacia |  |
| 1.14.13.24 | 0.13 | - |
O2 | - |
Burkholderia cepacia | |
| 1.14.13.24 | 0.19 | - |
3-hydroxybenzoate | - |
Burkholderia cepacia | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.24 | Burkholderia cepacia | - |
- |
- |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.13.24 | 3-hydroxybenzoate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O | mechanism | Burkholderia cepacia |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.24 | 3-hydroxybenzoate + NADH + O2 | - |
Burkholderia cepacia | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.24 | 23 | - |
assay at | Burkholderia cepacia |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.24 | NADH | - |
Burkholderia cepacia | |
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Release 2023.1 (January 2023)
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