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Literature summary extracted from

  • Lloyd, J.S.; Bhambra, A.; Murrell, J.C.; Dalton, H.
    Inactivation of the regulatory protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath) by proteolysis can be overcome by a Gly to Gln modification (1997), Eur. J. Biochem., 248, 72-79.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.25 expression of component protein B of sMMO as glutathione-S-transferase fusion protein in Escherichia coli Methylococcus capsulatus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.25 G13Q enhanced temperature stability compared to wild-type, site-directed mutagenesis Methylococcus capsulatus
1.14.13.25 G13Q sMMO, alteration of a cleavage site in component protein B Methylococcus capsulatus
1.14.13.25 additional information native parallel occurence of full length and 2 N-terminal truncated forms of regulatory component protein B of sMMO, truncated forms are inactive Methylococcus capsulatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.25 cytoplasm cytoplasmatic, soluble enzyme form termed sMMO Methylococcus capsulatus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.25 15850
-
 component protein B of sMMO, mass spectrometry Methylococcus capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylococcus capsulatus
-
 enzyme form sMMO
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.25 recombinant component protein B of sMMO as glutathione-S-transferase fusion protein from Escherichia coli Methylococcus capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.25 additional information
-
 recombinant shows higher activity than the wild-type Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 methane + NAD(P)H + O2
-
 Methylococcus capsulatus methanol + NAD(P)+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.25 MMO
-
 Methylococcus capsulatus
1.14.13.25 pMMO particulate, membrane-bound enzyme form Methylococcus capsulatus
1.14.13.25 sMMO soluble, cytoplasmic enzyme form Methylococcus capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NADH
-
 Methylococcus capsulatus