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Literature summary extracted from

  • Woodland, M.P.; Dalton, H.
    Purification and characterization of component A of the methane monooxygenase from Methylococcus capsulatus (Bath) (1984), J. Biol. Chem., 259, 53-59.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.25 Iron 2.3 mol Fe per mol protein Methylococcus capsulatus
1.14.13.25 Zn2+ 0.2-0.5 mol zinc per mol protein Methylococcus capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.25 additional information
-
 see under subunits: molecular weights of the subunits of components Methylococcus capsulatus
1.14.13.25 17000
-
 component A: 2 * 54000 alpha + 2 * 42000 beta + 2 * 17000 gamma, SDS-PAGE and analytical ultracentrifugation Methylococcus capsulatus
1.14.13.25 42000
-
 component A: 2 * 54000 alpha + 2 * 42000 beta + 2 * 17000 gamma, SDS-PAGE and analytical ultracentrifugation Methylococcus capsulatus
1.14.13.25 54000
-
 component A: 2 * 54000 alpha + 2 * 42000 beta + 2 * 17000 gamma, SDS-PAGE and analytical ultracentrifugation Methylococcus capsulatus
1.14.13.25 210000
-
 component A, analytical ultracentrifugation Methylococcus capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylococcus capsulatus
-
 Bath
-
1.14.13.25 Methylococcus capsulatus Bath
-
 Bath
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.25 component A Methylococcus capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.25 0.072
-
 component A Methylococcus capsulatus

Storage Stability

EC Number Storage Stability Organism
1.14.13.25 -20°C, 50% glycerol, component A, stable for several weeks Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 methane + NAD(P)H + O2
-
 Methylococcus capsulatus methanol + NAD(P)+ + H2O
-
 ?
1.14.13.25 methane + NAD(P)H + O2
-
 Methylococcus capsulatus Bath methanol + NAD(P)+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.25 ? component A: 2 * 54000 alpha + 2 * 42000 beta + 2 * 17000 gamma, SDS-PAGE and analytical ultracentrifugation Methylococcus capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NADH
-
 Methylococcus capsulatus