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Literature summary extracted from
- Methane monooxygenase from Methylosinus trichosporium OB3b (1990), Methods Enzymol., 188, 191-202.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.25 | 0.025 | - |
methane | - |
Methylosinus trichosporium |  |
| 1.14.13.25 | 0.035 | - |
furan | - |
Methylosinus trichosporium | |
| 1.14.13.25 | 0.05 | - |
NADH | - |
Methylosinus trichosporium | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.25 | Iron | 4.3 mol Fe per mol enzyme | Methylosinus trichosporium | |
| 1.14.13.25 | Iron | 2 mol Fe per mol enzyme | Methylosinus trichosporium | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | additional information | - |
see under subunits: molecular weights of the subunits of components | Methylosinus trichosporium |
| 1.14.13.25 | additional information | - |
enzyme system consists of 3 protein components A, B, C | Methylosinus trichosporium |
| 1.14.13.25 | 15100 | - |
component B, gel filtration | Methylosinus trichosporium |
| 1.14.13.25 | 22700 | - |
component A: 2 * 54400 alpha, 2 * 43000 beta + 2 * 22700 gamma, sedimentation velocity, SDS-PAGE, amino acid analysis | Methylosinus trichosporium |
| 1.14.13.25 | 38300 | 38400 | component C: reductase, gel filtration | Methylosinus trichosporium |
| 1.14.13.25 | 43000 | - |
component A: 2 * 54400 alpha, 2 * 43000 beta + 2 * 22700 gamma, sedimentation velocity, SDS-PAGE, amino acid analysis | Methylosinus trichosporium |
| 1.14.13.25 | 54400 | - |
component A: 2 * 54400 alpha, 2 * 43000 beta + 2 * 22700 gamma, sedimentation velocity, SDS-PAGE, amino acid analysis | Methylosinus trichosporium |
| 1.14.13.25 | 241000 | 246000 | protein A hydroxylase, gel filtration | Methylosinus trichosporium |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.25 | methane + NAD(P)H + O2 | Methylosinus trichosporium | - |
methanol + NAD(P)+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.25 | Methylosinus trichosporium | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.25 | improved purification protocol using stabilizing agents | Methylosinus trichosporium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | 26.1 | - |
purified enzyme, substrate propene | Methylosinus trichosporium |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.13.25 | -80°C, more than 1 year | Methylosinus trichosporium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.25 | furan + NAD(P)H + O2 | - |
Methylosinus trichosporium | ? | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + O2 | - |
Methylosinus trichosporium | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | propene + NAD(P)H + O2 | - |
Methylosinus trichosporium | 1,2-epoxypropane + NAD(P)+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.25 | ? | component A: 2 * 54400 alpha, 2 * 43000 beta + 2 * 22700 gamma, sedimentation velocity, SDS-PAGE, amino acid analysis | Methylosinus trichosporium |
| 1.14.13.25 | More | see under molecular weight for the size of the protein components | Methylosinus trichosporium |
| 1.14.13.25 | More | enzyme system consists of 3 protein components A, B, C | Methylosinus trichosporium |
| 1.14.13.25 | More | component A is a hydroxylase | Methylosinus trichosporium |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.25 | 3.7 | - |
methane | - |
Methylosinus trichosporium | |
| 1.14.13.25 | 4.4 | - |
Propene | - |
Methylosinus trichosporium | |
| 1.14.13.25 | 7.6 | - |
furan | - |
Methylosinus trichosporium | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | 7.5 | - |
furan, propene | Methylosinus trichosporium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.25 | FAD | protein C, reductase component: contains 1 mol FAD per mol protein | Methylosinus trichosporium | |
| 1.14.13.25 | NADH | - |
Methylosinus trichosporium | |
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