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Literature summary extracted from

  • Stirling, D.I.; Dalton, H.
    Properties of the methane mono-oxygenase from extracts of Methylosinus trichosporium OB3b and evidence for its similarity to the enzyme from Methylococcus capsulatus (Bath) (1979), Eur. J. Biochem., 96, 205-212.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.13.25 instability of enzyme in crude extract Methylococcus capsulatus
1.14.13.25 instability of enzyme in crude extract Methylosinus trichosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.25 2,4-Dichloro-(6-phenylphenoxy)ethylamine hydrochloride no inhibition Methylosinus trichosporium
1.14.13.25 8-hydroxyquinoline
-
 Methylosinus trichosporium
1.14.13.25 Acetylene
-
 Methylococcus capsulatus
1.14.13.25 Acetylene
-
 Methylosinus trichosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.25 cytoplasm
-
 Methylococcus capsulatus 5737
-
1.14.13.25 cytoplasm
-
 Methylosinus trichosporium 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.25 additional information
-
 see under subunits: molecular weights of the subunits of components Methylococcus capsulatus
1.14.13.25 additional information
-
 see under subunits: molecular weights of the subunits of components Methylosinus trichosporium
1.14.13.25 additional information
-
 enzyme system consists of 3 protein components A, B, C Methylococcus capsulatus
1.14.13.25 additional information
-
 enzyme system consists of 3 protein components A, B, C Methylosinus trichosporium

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylococcus capsulatus
-
 Bath
-
1.14.13.25 Methylococcus capsulatus Bath
-
 Bath
-
1.14.13.25 Methylosinus trichosporium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 methane + NAD(P)H + O2
-
 Methylococcus capsulatus methanol + NAD(P)+ + H2O
-
 ?
1.14.13.25 methane + NAD(P)H + O2
-
 Methylosinus trichosporium methanol + NAD(P)+ + H2O
-
 ?
1.14.13.25 methane + NAD(P)H + O2
-
 Methylococcus capsulatus Bath methanol + NAD(P)+ + H2O
-
 ?
1.14.13.25 propene + NAD(P)H + O2
-
 Methylococcus capsulatus 1,2-epoxypropane + NAD(P)+ + H2O
-
 ?
1.14.13.25 propene + NAD(P)H + O2
-
 Methylosinus trichosporium 1,2-epoxypropane + NAD(P)+ + H2O
-
 ?
1.14.13.25 propene + NAD(P)H + O2
-
 Methylococcus capsulatus Bath 1,2-epoxypropane + NAD(P)+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.25 ?
-
 Methylococcus capsulatus
1.14.13.25 ?
-
 Methylosinus trichosporium
1.14.13.25 More see under molecular weight for the size of the protein components Methylococcus capsulatus
1.14.13.25 More see under molecular weight for the size of the protein components Methylosinus trichosporium
1.14.13.25 More enzyme system consists of 3 protein components A, B, C Methylococcus capsulatus
1.14.13.25 More enzyme system consists of 3 protein components A, B, C Methylosinus trichosporium
1.14.13.25 More component A is a hydroxylase Methylococcus capsulatus
1.14.13.25 More component A is a hydroxylase Methylosinus trichosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NADH
-
 Methylococcus capsulatus
1.14.13.25 NADH
-
 Methylosinus trichosporium