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Literature summary extracted from
- Purification and properties of the methane mono-oxygenase enzyme system from Methylosinus trichosporium OB3b (1977), Biochem. J., 161, 333-344.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.13.25 | no component of the enzyme is stable to freezing | Methylosinus trichosporium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.25 | 2,4-Dichloro-(6-phenylphenoxy)ethylamine hydrochloride | - |
Methylosinus trichosporium |  |
| 1.14.13.25 | 2,4-Dichloro-(6-phenylphenoxy)ethyldiethylamine | - |
Methylosinus trichosporium | |
| 1.14.13.25 | 2-mercaptoethanol | - |
Methylosinus trichosporium | |
| 1.14.13.25 | 3-Amino-1,2,4-triazole | - |
Methylosinus trichosporium | |
| 1.14.13.25 | Allylthiourea | - |
Methylosinus trichosporium | |
| 1.14.13.25 | Dimercaptopropanol | - |
Methylosinus trichosporium | |
| 1.14.13.25 | dithiothreitol | - |
Methylosinus trichosporium | |
| 1.14.13.25 | KCN | - |
Methylosinus trichosporium | |
| 1.14.13.25 | o-phenanthroline | - |
Methylosinus trichosporium | |
| 1.14.13.25 | SKF 525A | - |
Methylosinus trichosporium | |
| 1.14.13.25 | Thioacetamide | - |
Methylosinus trichosporium | |
| 1.14.13.25 | Thiosemicarbazide | - |
Methylosinus trichosporium | |
| 1.14.13.25 | Thiourea | - |
Methylosinus trichosporium | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.25 | 0.066 | - |
methane | - |
Methylosinus trichosporium | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.25 | Cu2+ | copper-containing protein component contains one copper atom per molecule | Methylosinus trichosporium | |
| 1.14.13.25 | Cu2+ | cytochrome component contains 0.3-0.8 atoms copper per molecule | Methylosinus trichosporium | |
| 1.14.13.25 | Iron | cytochrome component contains 1 atom iron per molecule | Methylosinus trichosporium | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | additional information | - |
see under subunits: molecular weights of the subunits of components | Methylosinus trichosporium |
| 1.14.13.25 | additional information | - |
3 components: 1 soluble CO-binding cytochrome c, 1 copper-containing protein, and 1 small protein, SDS-PAGE | Methylosinus trichosporium |
| 1.14.13.25 | additional information | - |
enzyme system consists of 3 protein components A, B, C | Methylosinus trichosporium |
| 1.14.13.25 | 9400 | - |
component: small protein, native PAGE | Methylosinus trichosporium |
| 1.14.13.25 | 13000 | - |
component: CO-binding cytochrome c, native PAGE | Methylosinus trichosporium |
| 1.14.13.25 | 47000 | - |
component: copper-containing protein, native PAGE | Methylosinus trichosporium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.25 | Methylosinus trichosporium | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.25 | 3 components: a soluble CO-binding cytochrome c, a copper-containing protein, another small protein | Methylosinus trichosporium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | additional information | - |
- |
Methylosinus trichosporium |
| 1.14.13.25 | 6 | - |
- |
Methylosinus trichosporium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.25 | butane + NAD(P)H + O2 | - |
Methylosinus trichosporium | 1-butanol + 2-butanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | CO + NAD(P)H + O2 | - |
Methylosinus trichosporium | CO2 + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | dimethyl ether + NAD(P)H + O2 | no activity | Methylosinus trichosporium | methanol + formaldehyde + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | ethane + NAD(P)H + O2 | - |
Methylosinus trichosporium | ethanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + O2 | - |
Methylosinus trichosporium | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | propane + NAD(P)H + O2 | - |
Methylosinus trichosporium | 1-propanol + 2-propanol + NAD(P)+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.25 | ? | - |
Methylosinus trichosporium |
| 1.14.13.25 | More | see under molecular weight for the size of the protein components | Methylosinus trichosporium |
| 1.14.13.25 | More | enzyme system consists of 3 protein components A, B, C | Methylosinus trichosporium |
| 1.14.13.25 | More | 3 components: 1 soluble CO-binding cytochrome c, 1 copper-containing protein, and 1 small protein, SDS-PAGE | Methylosinus trichosporium |
| 1.14.13.25 | More | component A is a hydroxylase | Methylosinus trichosporium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | 6.9 | 7 | - |
Methylosinus trichosporium |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | 6.4 | 7.4 | pH 6.4: about 20% of activity maximum, pH 7.4: about 25% of activity maximum | Methylosinus trichosporium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.25 | cytochrome c | one of three protein components is a soluble CO-binding cytochrome c | Methylosinus trichosporium | |
| 1.14.13.25 | NADH | - |
Methylosinus trichosporium | |
| 1.14.13.25 | NADPH | - |
Methylosinus trichosporium | |
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