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Literature summary extracted from

  • Mitra, D.; Vaidyanathan, C.S.
    A new 4-nitrophenol 2-hydroxylase from a Nocardia sp (1984), Biochem. Int., 8, 609-615.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.13.29 dialysis of crude enzyme extract, complete loss of activity Nocardia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.29 catalase 30% inhibition at 1000 units Nocardia sp.
1.14.13.29 Co2+ slight effect, crude enzyme extract Nocardia sp.
1.14.13.29 Cu2+ 94% inhibition at 1 mM,crude enzyme extract Nocardia sp.
1.14.13.29 Fe2+ slight effect, crude enzyme extract Nocardia sp.
1.14.13.29 Fe3+ slight effect, crude enzyme extract Nocardia sp.
1.14.13.29 Hg2+ 63% inhibition at 1 mM, crude enzyme extract Nocardia sp.
1.14.13.29 Horseradish peroxidase complete inhibition at 25 units Nocardia sp.
1.14.13.29 additional information inhibition at high ionic strength of all common buffers and salts e.g. phosphate, Tris, KCl, (NH4)2SO4, 60% inhibition above 300 mM, crude enzyme extract Nocardia sp.
1.14.13.29 N-Methylmaleimide 84% inhibition at 5 mM Nocardia sp.
1.14.13.29 Ni2+ slight effect, crude enzyme extract Nocardia sp.
1.14.13.29 p-chloromercuribenzoate 81% inhibition at 1 mM Nocardia sp.
1.14.13.29 Sn2+ 57% inhibition at 1 mM,crude enzyme extract Nocardia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.29 0.0083
-
 4-nitrocatechol
-
 Nocardia sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.29 cytosol
-
 Nocardia sp. 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.29 Nocardia sp.
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.14.13.29 -20°C, 85% loss of activity after 10 days Nocardia sp.
1.14.13.29 0-4°C, 30% loss of activity after 7 days, 70% loss of activity after 14 days, crude enzyme extract Nocardia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.29 3-nitrophenol + NADH + O2
-
 Nocardia sp. ?
-
 ?
1.14.13.29 4-nitrophenol + NADH + O2 NADPH: 50% of the activity with NADH Nocardia sp. 4-nitrocatechol + NAD+ + H2O
-
 ?
1.14.13.29 chlorzoxazone + NADH + O2
-
 Nocardia sp. ?
-
 ?
1.14.13.29 additional information no substrates: 2-nitrophenol, 2,4-dinitrophenol Nocardia sp. ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.29 40
-
 crude enzyme extract Nocardia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.29 7.3
-
 crude enzyme extract Nocardia sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.29 FAD flavoprotein, FMN cannot replace FAD Nocardia sp.
1.14.13.29 NADH
-
 Nocardia sp.
1.14.13.29 NADPH 50% of the activity with NADH Nocardia sp.