EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.9 | Br- | retards the oxidative half-reaction | Pseudomonas putida | |
1.14.14.9 | Cl- | retards the oxidative half-reaction | Pseudomonas putida | |
1.14.14.9 | F- | retards the oxidative half-reaction | Pseudomonas putida | |
1.14.14.9 | I- | retards the oxidative half-reaction | Pseudomonas putida | |
1.14.14.9 | N3- | retards the oxidative half-reaction | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | Pseudomonas putida | - |
3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.9 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.9 | - |
Pseudomonas putida |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.14.9 | 4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O | two-protein enzyme: the flavoprotein catalyzes the non-productive oxidation of NADH without substrate hydroxylation and the coupling protein is an absolute requirement for productive hydroxylation. Reaction mechanism and formation of 3 flavin-oxygen intermediates are studied | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.9 | 3-(4-hydroxyphenyl)propionate + ? | - |
Pseudomonas putida | 3-(3,4-dihydroxy)phenylpropionate + ? | - |
? | |
1.14.14.9 | 4-aminophenylacetate + ? | - |
Pseudomonas putida | 4-amino-3-hydroxyphenylacetate + ? | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | - |
Pseudomonas putida | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.9 | FAD | flavoprotein monooxygenase, absolute requirement | Pseudomonas putida |