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Literature summary extracted from

  • Barbour, M.G.; Bayly, R.C.
    Regulation of the meta-cleavage of 4-hydroxyphenylacetic acid by Pseudomonas putida (1977), Biochem. Biophys. Res. Commun., 76, 565-571.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.9 additional information hydroxylase deficient mutant P23X6 created by exposure to ethylmethane sulfonate Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.15 3,4-dihydroxyphenylacetate + O2 Pseudomonas putida induced by 3,4-dihydroxyphenylacetate 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 Pseudomonas putida degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.15 Pseudomonas putida
-
 no activity in strain P23X28
-
1.13.11.15 Pseudomonas putida
-
 wild-type strain P23X1
-
1.14.14.9 Pseudomonas putida
-
 mutant P23X1
-
1.14.14.9 Pseudomonas putida
-
 P23X1, NCIB 9865
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.14.9 4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O genes encoding the first four enzymes of the 4-hydroxyphenylacetic acid meta-pathway exist as two operons, one containing the hydroxylase gene while the other contains the other three Pseudomonas putida
a
1.14.14.9 4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O enzymes of 4-hydroxyphenylacetic acid meta-cleavage pathway are sythesized following at least two sequential inductive events Pseudomonas putida
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.9 additional information
-
 no functional enzyme and activity detected for mutant, wild-type shows activity only if exposed to 4-hydroxyphenylacetate Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Pseudomonas putida 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2 induced by 3,4-dihydroxyphenylacetate Pseudomonas putida 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis Pseudomonas putida 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ir
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway Pseudomonas putida 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?