Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Barbour, M.G.; Bayly, R.C.
    Regulation of the 4-hydroxyphenylacetic acid meta-cleavage pathway in an Acinetobacter sp (1977), Biochem. Biophys. Res. Commun., 79, 663-670.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.9 additional information 3B-3 mutant deficient in the metabolism of 4-hydroxyphenylacetic acid are created by exposure to N-methyl-N'-nitro-N-nitrosoguanidine Acinetobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.15 3,4-dihydroxyphenylacetate + O2 Acinetobacter sp. enzyme is involved in the meta-cleavage pathway for the degradation of 4-hydroxyphenylacetic acid 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2 Acinetobacter sp. 3B-1 enzyme is involved in the meta-cleavage pathway for the degradation of 4-hydroxyphenylacetic acid 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 Acinetobacter sp.
-
 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 Acinetobacter sp. 3B-1
-
 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.15 Acinetobacter sp.
-
-
-
1.13.11.15 Acinetobacter sp. 3B-1
-
-
-
1.14.14.9 Acinetobacter sp.
-
 wild-type strain 3B-1 and mutant 3B-3
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.14.9 4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O enzymes of 4-hydroxyphenylacetic acid meta-cleavage pathway are sythesized following at least two sequential inductive events Acinetobacter sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.9 additional information
-
 no functional enzyme and activity detected for mutant, wild-type shows activity only if exposed to 4-hydroxyphenylacetate Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Acinetobacter sp. 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2 enzyme is involved in the meta-cleavage pathway for the degradation of 4-hydroxyphenylacetic acid Acinetobacter sp. 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Acinetobacter sp. 3B-1 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2 enzyme is involved in the meta-cleavage pathway for the degradation of 4-hydroxyphenylacetic acid Acinetobacter sp. 3B-1 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2
-
 Acinetobacter sp. 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis Acinetobacter sp. 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ir
1.14.14.9 4-hydroxyphenylacetate + NADH + O2
-
 Acinetobacter sp. 3B-1 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2 enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis Acinetobacter sp. 3B-1 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ir