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Literature summary extracted from
- Purification and properties of 4-hydroxyphenylacetic acid 3-hydroxylase from Pseudomonas putida (1988), Biochem. Biophys. Res. Commun., 154, 537-543.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.9 | Carbohydrates | stimulate synthesis of enzyme insignificantly | Pseudomonas putida | |
| 1.14.14.9 | additional information | not activated by beta-mercaptoethanol, glutathione and dithiothreiotol | Pseudomonas putida |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.14.9 | unstable | Pseudomonas putida |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.9 | Cibacron blue F3GA | dye | Pseudomonas putida |  |
| 1.14.14.9 | Cu2+ | - |
Pseudomonas putida | |
| 1.14.14.9 | Hg2+ | - |
Pseudomonas putida | |
| 1.14.14.9 | additional information | not inhibited by Mn2+, Mg2+, Fe3+ and Fe2+ | Pseudomonas putida | |
| 1.14.14.9 | N-ethylmaleimide | 48% inhibition at 1 mM | Pseudomonas putida | |
| 1.14.14.9 | p-chloromercuribenzoate | 92% inhibition at 1 mM | Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.9 | 0.059 | - |
NADH | - |
Pseudomonas putida | |
| 1.14.14.9 | 0.2 | - |
4-hydroxyphenylacetate | - |
Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.9 | 91000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | Pseudomonas putida | key enzyme in pathway of degradation of phenylalanine, tyrosine and other aromatic amines | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.9 | Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.9 | ammonium sulfate precipitation, gel filtration, affinity chromatography | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.9 | additional information | - |
maximal in enzymes of the late log phase | Pseudomonas putida |
| 1.14.14.9 | 6.5 | - |
- |
Pseudomonas putida |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.14.9 | 4°C, 15-20 days | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.9 | 3-hydroxyphenylacetate + NADH + O2 | - |
Pseudomonas putida | 3,4-hydroxyphenylacetate + NAD+ + H2O | - |
? | |
| 1.14.14.9 | 4-fluorophenylacetate + NADH + O2 | - |
Pseudomonas putida | 4-fluoro-3-hydroxyphenylacetate + NAD+ + H2O | - |
? | |
| 1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | - |
Pseudomonas putida | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
| 1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | key enzyme in pathway of degradation of phenylalanine, tyrosine and other aromatic amines | Pseudomonas putida | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.9 | dimer | 2 * x, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.9 | 30 | - |
assay at | Pseudomonas putida |
| 1.14.14.9 | 45 | - |
- |
Pseudomonas putida |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.9 | 4 | - |
15-20 days | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.9 | 7.5 | - |
- |
Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.9 | FAD | flavoprotein monooxygenase, absolute requirement | Pseudomonas putida | |
| 1.14.14.9 | NADH | absolute requirement | Pseudomonas putida | |
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