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Literature summary extracted from

  • Adachi, K.; Takeda, Y.; Senoh, S.; Kita, H.
    Metabolism of p-hydroxyphenylacetic acid in Pseudomonas ovalis (1964), Biochim. Biophys. Acta, 93, 483-493.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.9 o-phenanthroline complete inhibition at 1 mM Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.15 Pseudomonas putida
-
-
-
1.14.14.9 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.15 partial Pseudomonas putida
1.14.14.9 protamine sulfate and ammonium sulfate Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.15 culture condition:p-hydroxyphenylacetic acid-grown cell
-
 Pseudomonas putida
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Pseudomonas putida 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + O2
-
 Pseudomonas putida 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.9 30
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.9 7.2
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.9 NADH can not be replaced by NADPH Pseudomonas putida