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Literature summary extracted from

  • Kadiyala, V.; Spain, J.C.
    A two-component monooxygenase catalyzes both the hydroxylation of p-nitrophenol and the oxidative release of nitrite from 4-nitrocatechol in Bacillus sphaericus JS905 (1998), Appl. Environ. Microbiol., 64, 2479-2484.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.166 2-mercaptoethanol
-
Lysinibacillus sphaericus
1.14.13.166 Methimazole 58% inhibition at 0.5 mM Lysinibacillus sphaericus
1.14.13.166 additional information alpha-naphthoflavone, miconazole, and metyrapone at 0.5 mM have very little effect (less than 5% inhibition) on enzyme activity Lysinibacillus sphaericus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.166 Mg2+ magnesium ions enhance the enzyme activity Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.31 2-nitrophenol + NADPH + O2 Lysinibacillus sphaericus
-
?
-
?
1.14.13.31 2-nitrophenol + NADPH + O2 Lysinibacillus sphaericus JS905
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.31 Lysinibacillus sphaericus
-
-
-
1.14.13.31 Lysinibacillus sphaericus JS905
-
-
-
1.14.13.166 Lysinibacillus sphaericus
-
-
-
1.14.13.166 Lysinibacillus sphaericus JS905
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.31 partial Lysinibacillus sphaericus
1.14.13.166 DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, and Sephacryl S-300 gel filtration Lysinibacillus sphaericus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.166 culture condition:4-nitrophenol-grown cell
-
Lysinibacillus sphaericus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.31 0.04
-
-
Lysinibacillus sphaericus
1.14.13.166 0.0013
-
cell extract, using 4-nitrocatechol as substrate, at pH 8.0 and 30°C Lysinibacillus sphaericus
1.14.13.166 0.0013
-
cell extract, using 4-nitrophenol as substrate, at pH 8.0 and 30°C Lysinibacillus sphaericus
1.14.13.166 0.038
-
after 28fold purification, using 4-nitrocatechol as substrate, at pH 8.0 and 30°C Lysinibacillus sphaericus
1.14.13.166 0.04
-
after 32fold purification, using 4-nitrophenol as substrate, at pH 8.0 and 30°C Lysinibacillus sphaericus

Storage Stability

EC Number Storage Stability Organism
1.14.13.166 . Lysinibacillus sphaericus
1.14.13.166 0°C, extracts prepared without added FAD, 48 h, 52% loss of activity Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.31 2-nitrophenol + NADPH + H+ + O2
-
Lysinibacillus sphaericus 3-nitrocatechol + NADP+ + H2O
-
?
1.14.13.31 2-nitrophenol + NADPH + H+ + O2
-
Lysinibacillus sphaericus JS905 3-nitrocatechol + NADP+ + H2O
-
?
1.14.13.31 2-nitrophenol + NADPH + O2
-
Lysinibacillus sphaericus ?
-
?
1.14.13.31 2-nitrophenol + NADPH + O2
-
Lysinibacillus sphaericus JS905 ?
-
?
1.14.13.31 4-nitroresorcinol + NADPH + O2
-
Lysinibacillus sphaericus ?
-
?
1.14.13.31 4-nitroresorcinol + NADPH + O2
-
Lysinibacillus sphaericus JS905 ?
-
?
1.14.13.31 additional information 2-component enzyme system comprising of a flavoprotein reductase and an oxygenase Lysinibacillus sphaericus ?
-
?
1.14.13.31 additional information 2-component enzyme system comprising of a flavoprotein reductase and an oxygenase Lysinibacillus sphaericus JS905 ?
-
?
1.14.13.166 4-nitrocatechol + NADH + H+ + O2
-
Lysinibacillus sphaericus 2-hydroxy-1,4-benzoquinone + nitrite + NAD+ + H2O
-
?
1.14.13.166 additional information NADPH does not support 4-nitrophenol or 4-nitrocatechol oxidation Lysinibacillus sphaericus ?
-
?
1.14.13.166 additional information NADPH does not support 4-nitrophenol or 4-nitrocatechol oxidation Lysinibacillus sphaericus JS905 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.166 PNP monooxygenase the enzyme system consists of two components that catalyze two consecutive monooxygenation reactions Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.166 8
-
in 50 mM Tris-HCl Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.31 FAD
-
Lysinibacillus sphaericus
1.14.13.31 NADH
-
Lysinibacillus sphaericus
1.14.13.31 NADPH
-
Lysinibacillus sphaericus
1.14.13.166 FAD required for full activity. The reductase component of the enzyme has a loosely bound FAD Lysinibacillus sphaericus