EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.166 | 2-mercaptoethanol | - |
Lysinibacillus sphaericus | |
1.14.13.166 | Methimazole | 58% inhibition at 0.5 mM | Lysinibacillus sphaericus | |
1.14.13.166 | additional information | alpha-naphthoflavone, miconazole, and metyrapone at 0.5 mM have very little effect (less than 5% inhibition) on enzyme activity | Lysinibacillus sphaericus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.166 | Mg2+ | magnesium ions enhance the enzyme activity | Lysinibacillus sphaericus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.31 | 2-nitrophenol + NADPH + O2 | Lysinibacillus sphaericus | - |
? | - |
? | |
1.14.13.31 | 2-nitrophenol + NADPH + O2 | Lysinibacillus sphaericus JS905 | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.31 | Lysinibacillus sphaericus | - |
- |
- |
1.14.13.31 | Lysinibacillus sphaericus JS905 | - |
- |
- |
1.14.13.166 | Lysinibacillus sphaericus | - |
- |
- |
1.14.13.166 | Lysinibacillus sphaericus JS905 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.31 | partial | Lysinibacillus sphaericus |
1.14.13.166 | DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, and Sephacryl S-300 gel filtration | Lysinibacillus sphaericus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.13.166 | culture condition:4-nitrophenol-grown cell | - |
Lysinibacillus sphaericus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.31 | 0.04 | - |
- |
Lysinibacillus sphaericus |
1.14.13.166 | 0.0013 | - |
cell extract, using 4-nitrocatechol as substrate, at pH 8.0 and 30°C | Lysinibacillus sphaericus |
1.14.13.166 | 0.0013 | - |
cell extract, using 4-nitrophenol as substrate, at pH 8.0 and 30°C | Lysinibacillus sphaericus |
1.14.13.166 | 0.038 | - |
after 28fold purification, using 4-nitrocatechol as substrate, at pH 8.0 and 30°C | Lysinibacillus sphaericus |
1.14.13.166 | 0.04 | - |
after 32fold purification, using 4-nitrophenol as substrate, at pH 8.0 and 30°C | Lysinibacillus sphaericus |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.13.166 | . | Lysinibacillus sphaericus |
1.14.13.166 | 0°C, extracts prepared without added FAD, 48 h, 52% loss of activity | Lysinibacillus sphaericus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.31 | 2-nitrophenol + NADPH + H+ + O2 | - |
Lysinibacillus sphaericus | 3-nitrocatechol + NADP+ + H2O | - |
? | |
1.14.13.31 | 2-nitrophenol + NADPH + H+ + O2 | - |
Lysinibacillus sphaericus JS905 | 3-nitrocatechol + NADP+ + H2O | - |
? | |
1.14.13.31 | 2-nitrophenol + NADPH + O2 | - |
Lysinibacillus sphaericus | ? | - |
? | |
1.14.13.31 | 2-nitrophenol + NADPH + O2 | - |
Lysinibacillus sphaericus JS905 | ? | - |
? | |
1.14.13.31 | 4-nitroresorcinol + NADPH + O2 | - |
Lysinibacillus sphaericus | ? | - |
? | |
1.14.13.31 | 4-nitroresorcinol + NADPH + O2 | - |
Lysinibacillus sphaericus JS905 | ? | - |
? | |
1.14.13.31 | additional information | 2-component enzyme system comprising of a flavoprotein reductase and an oxygenase | Lysinibacillus sphaericus | ? | - |
? | |
1.14.13.31 | additional information | 2-component enzyme system comprising of a flavoprotein reductase and an oxygenase | Lysinibacillus sphaericus JS905 | ? | - |
? | |
1.14.13.166 | 4-nitrocatechol + NADH + H+ + O2 | - |
Lysinibacillus sphaericus | 2-hydroxy-1,4-benzoquinone + nitrite + NAD+ + H2O | - |
? | |
1.14.13.166 | additional information | NADPH does not support 4-nitrophenol or 4-nitrocatechol oxidation | Lysinibacillus sphaericus | ? | - |
? | |
1.14.13.166 | additional information | NADPH does not support 4-nitrophenol or 4-nitrocatechol oxidation | Lysinibacillus sphaericus JS905 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.166 | PNP monooxygenase | the enzyme system consists of two components that catalyze two consecutive monooxygenation reactions | Lysinibacillus sphaericus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.166 | 8 | - |
in 50 mM Tris-HCl | Lysinibacillus sphaericus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.31 | FAD | - |
Lysinibacillus sphaericus | |
1.14.13.31 | NADH | - |
Lysinibacillus sphaericus | |
1.14.13.31 | NADPH | - |
Lysinibacillus sphaericus | |
1.14.13.166 | FAD | required for full activity. The reductase component of the enzyme has a loosely bound FAD | Lysinibacillus sphaericus |