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Literature summary extracted from

  • Subramanian, V.; Vaidyanathan, C.S.
    Anthranilate hydroxylase from Aspergillus niger: new type of NADPH-linked nonheme iron monooxygenase (1984), J. Bacteriol., 160, 651-655.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.35 1,10-phenanthroline 50% inhibition at 0.25 mM, 80% inhibition at 0.5 mM and 95% inhibition at 1 mM Aspergillus niger
1.14.13.35 2,2'-dipyridyl 50% inhibition at 0.5 mM, 80% inhibition at 1 mM Aspergillus niger
1.14.13.35 8-hydroxyquinoline 30% inhibition at 0.5 mM, 40% inhibition at 1 mM Aspergillus niger
1.14.13.35 additional information not: atebrin, aminopterin Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.35 Iron contains 2 gatom of non-heme iron per mol Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.35 42000
-
 2 * 42000, SDS-PAGE Aspergillus niger
1.14.13.35 89000
-
 gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.35 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.35 using protamine sulfate treatment, DEAE-cellulose treatment, ammonium sulfate precipitation, fractionation on Biogel P-100 column, successive negative adsorption on alumina-gel, tricalcium phosphate gel and DEAE-cellulose column, and positive adsorption on a DEAE-Sephadex A-50 column Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.35 0.193
-
-
 Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
1.14.13.35 -20°C, ammonium sulfate precipitate, stable for at least 3 months Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.35 anthranilate + NADPH + O2
-
 Aspergillus niger 2,3-dihydroxybenzoate + NADP+ + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.35 dimer 2 * 42000, SDS-PAGE Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.35 4
-
 half-life: 14 h Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.35 FAD flavoprotein Aspergillus niger
1.14.13.35 NADPH
-
 Aspergillus niger