Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lange, C.C.; Schneider, B.J.; Orser, C.S.
    Verification of the role of PCP 4-monooxygenase in chlorine elimination from pentachlorophenol by Flavobacterium sp. strain ATCC 39723 (1996), Biochem. Biophys. Res. Commun., 219, 146-149.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.50 pcpB gene, expression in Escherichia coli and Flavobacterium sp. ATCC 39723 of wild-type and isogenic mutant, DNA sequence analysis Flavobacterium sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.50 additional information targeted mutagenesis via allelic exchange, construction of isogenic mutant without PCP 4-monooxygenase activity and 2 cointegration mutants with slow activity Flavobacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.50 pentachlorophenol + 2 NADPH + H+ + O2 Flavobacterium sp. solitary oxygenolytic pathway for removal of the primary chlorine during PCP degradation, not a hydrolytic one tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
-
?
1.14.13.50 pentachlorophenol + 2 NADPH + H+ + O2 Flavobacterium sp. wild-type and mutant strains tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.50 Flavobacterium sp.
-
strain ATCC 39723
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.50 additional information broad substrate and catalytic reaction range Flavobacterium sp. ?
-
?
1.14.13.50 pentachlorophenol + 2 NADPH + H+ + O2 solitary oxygenolytic pathway for removal of the primary chlorine during PCP degradation, not a hydrolytic one Flavobacterium sp. tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
-
?
1.14.13.50 pentachlorophenol + 2 NADPH + H+ + O2 wild-type and mutant strains Flavobacterium sp. tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
-
?
1.14.13.50 pentachlorophenol + NADPH + H+ + O2
-
Flavobacterium sp. 2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.50 FAD
-
Flavobacterium sp.
1.14.13.50 NADPH requirement Flavobacterium sp.