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Literature summary extracted from

  • Xun, L.; Orser, C.S.
    Purification and properties of pentachlorophenol hydroxylase, a flavoprotein from Flavobacterium sp. strain ATCC 39723 [published erratum appears in J Bacteriol 1992 Aug;174(15):5176] (1991), J. Bacteriol., 173, 4447-4453.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.50
-
 Flavobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.50 0.03
-
 Pentachlorophenol
-
 Flavobacterium sp.
1.14.13.50 0.082
-
 NADPH
-
 Flavobacterium sp.
1.14.13.50 0.214
-
 NADH
-
 Flavobacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.50 additional information no requirement for divalent cations Flavobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.50 63000
-
 1 * 63000, SDS-PAGE, under certain conditions dimer and multimer conformations are also observed Flavobacterium sp.
1.14.13.50 66000
-
 gel filtration Flavobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.50 Flavobacterium sp.
-
 strain ATCC 39723
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.50
-
 Flavobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.50 additional information
-
-
 Flavobacterium sp.

Storage Stability

EC Number Storage Stability Organism
1.14.13.50 -20°C, as ammonium sulfate precipitate or in solution at concentration greater than 1 mg/ml, 2 months Flavobacterium sp.
1.14.13.50 4°C, 50% loss of activity after 1 week Flavobacterium sp.
1.14.13.50 4°C, in cell extract or as 40-60% fraction of ammonium sulfate precipitation, total loss of activity after 3 days Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.50 pentachlorophenol + NADH + H+ + O2
-
 Flavobacterium sp. 2,3,5,6-tetrachloro-1,4-benzoquinone + NAD+ + chloride + H2O
-
 r
1.14.13.50 pentachlorophenol + NADPH + H+ + O2
-
 Flavobacterium sp. 2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
 ?
1.14.13.50 triiodophenol + NADPH + O2
-
 Flavobacterium sp. diiodohydroquinone + iodide + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.50 monomer 1 * 63000, SDS-PAGE, under certain conditions dimer and multimer conformations are also observed Flavobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.50 40
-
-
 Flavobacterium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.13.50 40 45 40°C: activity maximum, 45°C: above 75% of activity maximum, 50°C: inactive Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.50 7.5 8.5
-
 Flavobacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.50 6 8.5 pH 6.0: about 28% of activity maximum, pH 7.5-8.5: activity maximum Flavobacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.50 FAD 1 FAD per enzyme molecule Flavobacterium sp.
1.14.13.50 additional information dithiothreitol can not be used as electron donor Flavobacterium sp.
1.14.13.50 NADH can replace NADPH, less effective Flavobacterium sp.
1.14.13.50 NADPH 2 mol of NADPH per mol of halogenated substrate, preferred electron donor Flavobacterium sp.