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Literature summary extracted from

  • Peelen, S.; Rietjens, I.M.C.M.; van Berkel, W.J.H.; van Workum, W.A.T.; Vervoort, J.
    Fluorine-19 NMR study on the pH-dependent regioselectivity and rate of the ortho-hydroxylation of 3-fluorophenol by phenol hydroxylase from Trichosporon cutaneum. Implications for the reaction mechanism (1993), Eur. J. Biochem., 218, 345-353.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.7 Cutaneotrichosporon cutaneum
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.7 90% pure Cutaneotrichosporon cutaneum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.7 6
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Cutaneotrichosporon cutaneum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.7 3-fluorophenol + O2 + NADPH below pH 6.5 3-fluorophenol is preferentially hydroxylated at the C6 ortho position, at increasing pH the C2 ortho-hydroxylation becomes more predominant Cutaneotrichosporon cutaneum ?
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