Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • McGhie, E.J.; Littlechild, J.A.
    The purification and crystallization of 2,5-diketocamphane 1,2-monooxygenase and 3,6-diketocamphane 1,6-monooxygenase from Pseudomonas putida NCIMB 10007 (1996), Biochem. Soc. Trans., 24, 29S.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.14.108 crystal clusters, grown in 0.2 M CaCl2, 0.1 M HEPES and 30% PEG 400, X-ray analysis Pseudomonas putida
1.14.14.155 plate shaped crystals from 4.0 M sodium formate and 28% PEG 8000 in 0.2 M sodium acetate, X-ray analysis Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.108 additional information
-
 enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase Pseudomonas putida
1.14.14.108 36000
-
 NADH dehydrogenase component, SDS-PAGE Pseudomonas putida
1.14.14.108 39000 40000 oxygenating component, gel filtration Pseudomonas putida
1.14.14.155 additional information
-
 enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase Pseudomonas putida
1.14.14.155 36000
-
 NADH dehydrogenase component, SDS-PAGE Pseudomonas putida
1.14.14.155 38000
-
 oxygenating component, gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.108 (+)-bornane-2,5-dione + FMNH2 + O2 Pseudomonas putida inducible by growth on racemic camphor ?
-
 ?
1.14.14.108 (+)-bornane-2,5-dione + FMNH2 + O2 Pseudomonas putida NCIMB 10007 inducible by growth on racemic camphor ?
-
 ?
1.14.14.155 (-)-bornane-2,5-dione + FMNH2 + O2 Pseudomonas putida inducible by growth on racemic camphor ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.108 Pseudomonas putida
-
-
-
1.14.14.108 Pseudomonas putida NCIMB 10007
-
-
-
1.14.14.155 Pseudomonas putida
-
-
-
1.14.14.155 Pseudomonas putida NCIMB 10007
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.108 both components Pseudomonas putida
1.14.14.155 both components Pseudomonas putida

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.14.108 (+)-bornane-2,5-dione + FMNH2 + O2 = (+)-5-oxo-1,2-campholide + FMN + H2O mechanism Pseudomonas putida
a
1.14.14.108 (+)-bornane-2,5-dione + FMNH2 + O2 = (+)-5-oxo-1,2-campholide + FMN + H2O belongs to group of Bayer-Villiger monooxygenases of the NADH plus FMN-dependent type 2 enzymes Pseudomonas putida
a
1.14.14.155 (-)-bornane-2,5-dione + reduced rubredoxin + O2 + FMNH2 = (-)-5-oxo-1,2-campholide + FMN + H2O mechanism Pseudomonas putida
a
1.14.14.155 (-)-bornane-2,5-dione + reduced rubredoxin + O2 + FMNH2 = (-)-5-oxo-1,2-campholide + FMN + H2O belongs to group of Bayer-Villiger monooxygenases of the NADH plus FMN-dependent type 2 enzymes Pseudomonas putida
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.108 (+)-bornane-2,5-dione + FMNH2 + O2 i.e. 2,5-diketocamphane Pseudomonas putida 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O product is an unstable lactone-intermediate and forms spontaneously 2-oxo-DELTA3-4,5,5-trimethylcyclopentenyl acetic acid ?
1.14.14.108 (+)-bornane-2,5-dione + FMNH2 + O2 i.e. 2,5-diketocamphane Pseudomonas putida NCIMB 10007 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O product is an unstable lactone-intermediate and forms spontaneously 2-oxo-DELTA3-4,5,5-trimethylcyclopentenyl acetic acid ?
1.14.14.108 (+)-bornane-2,5-dione + FMNH2 + O2 inducible by growth on racemic camphor Pseudomonas putida ?
-
 ?
1.14.14.108 (+)-bornane-2,5-dione + FMNH2 + O2 inducible by growth on racemic camphor Pseudomonas putida NCIMB 10007 ?
-
 ?
1.14.14.108 (+)-bornanone + FMNH2 + O2
-
 Pseudomonas putida 1,2-campholide + FMN + H2O
-
 ?
1.14.14.108 (+)-bornanone + FMNH2 + O2
-
 Pseudomonas putida NCIMB 10007 1,2-campholide + FMN + H2O
-
 ?
1.14.14.108 additional information rubredoxin not mentioned Pseudomonas putida ?
-
 ?
1.14.14.108 additional information rubredoxin not mentioned Pseudomonas putida NCIMB 10007 ?
-
 ?
1.14.14.155 (-)-bornane-2,5-dione + FMNH2 + O2 6-dione i.e. 3,6-diketocamphane Pseudomonas putida 1,8,8-trimethyl-2-oxabicyclo[3.2.1]octane-3,6-dione + FMN + H2O i.e. cyclopentaneacetic acid, 3-hydroxy-2,2,3-trimethyl-5-oxo, delta-lactone, i.e. 3,4,4-trimethyl-6-carboxy-methyl-DELTA3-cyclopentenone, product is an unstable lactone-intermediate and forms spontaneously (2,2-dimethyl-5-oxo-cyclopent-3-en-1-yl)acetic acid, i.e. 2-oxo-DELTA3-4,5,5-trimethylcyclopentenyl acetic acid ?
1.14.14.155 (-)-bornane-2,5-dione + FMNH2 + O2 inducible by growth on racemic camphor Pseudomonas putida ?
-
 ?
1.14.14.155 (-)-bornanone + FMNH2 + O2 i.e. (-)-camphor Pseudomonas putida ?
-
 ?
1.14.14.155 additional information rubredoxin not mentioned Pseudomonas putida ?
-
 ?
1.14.14.155 additional information rubredoxin not mentioned Pseudomonas putida NCIMB 10007 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.108 More enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase Pseudomonas putida
1.14.14.155 monomer 1 * 38000, oxygenating component, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.14.155 3,6-diketocamphane-monooxygenase
-
 Pseudomonas putida