EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.108 | cytoplasm | NADH dehydrogenase component forming a loose complex with the oxygenating component | Pseudomonas putida | 5737 | - |
1.14.14.155 | cytoplasm | NADH dehydrogenase component forming a loosely complex with the oxygenating component | Pseudomonas putida | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.108 | additional information | - |
enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase | Pseudomonas putida |
1.14.14.108 | additional information | - |
enzyme components form a very loose complex | Pseudomonas putida |
1.14.14.108 | 44000 | - |
about, oxygenating component, native PAGE | Pseudomonas putida |
1.14.14.155 | additional information | - |
enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase | Pseudomonas putida |
1.14.14.155 | additional information | - |
enzyme components form a very loose complex | Pseudomonas putida |
1.14.14.155 | 40300 | - |
oxygenating component, native PAGE | Pseudomonas putida |
1.14.14.155 | 76000 | - |
both components, ultracentrifugal analysis | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.108 | Pseudomonas putida | - |
- |
- |
1.14.14.108 | Pseudomonas putida | - |
inducible by (+)-camphor | - |
1.14.14.108 | Pseudomonas putida C1 / ATCC 17453 | - |
- |
- |
1.14.14.155 | Pseudomonas putida | - |
inducible by (-)-camphor | - |
1.14.14.155 | Pseudomonas putida | - |
strain ATCC 17453 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.108 | both components | Pseudomonas putida |
1.14.14.155 | oxygenating component | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.14.108 | additional information | - |
no enzyme assay possible in crude extract due to competition with other enzymes for NADH | Pseudomonas putida |
1.14.14.108 | 0.335 | - |
substrate (+)-camphor | Pseudomonas putida |
1.14.14.155 | 0.244 | - |
substrate (-)-camphor | Pseudomonas putida |
1.14.14.155 | 300 | - |
purified enzyme component NADH dehydrogenase | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.108 | (+)-bornane-2,5-dione + FMNH2 + O2 | i.e. 2,5-diketocamphane | Pseudomonas putida | 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O | - |
? | |
1.14.14.108 | (+)-bornane-2,5-dione + FMNH2 + O2 | i.e. 2,5-diketocamphane | Pseudomonas putida C1 / ATCC 17453 | 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O | - |
? | |
1.14.14.108 | (+)-bornanone + FMNH2 + O2 | (+)-camphor | Pseudomonas putida | 1,2-campholide + FMN + H2O | - |
? | |
1.14.14.108 | (+)-bornanone + FMNH2 + O2 | (+)-camphor | Pseudomonas putida C1 / ATCC 17453 | 1,2-campholide + FMN + H2O | - |
? | |
1.14.14.108 | additional information | enzyme is associated with a NADH oxidase | Pseudomonas putida | ? | - |
? | |
1.14.14.108 | additional information | rubredoxin not mentioned | Pseudomonas putida | ? | - |
? | |
1.14.14.108 | additional information | no activity with (-)-camphor and other ketocamphanes that do not possess a keto group at position 2 | Pseudomonas putida | ? | - |
? | |
1.14.14.108 | additional information | enzyme is associated with a NADH oxidase | Pseudomonas putida C1 / ATCC 17453 | ? | - |
? | |
1.14.14.108 | additional information | rubredoxin not mentioned | Pseudomonas putida C1 / ATCC 17453 | ? | - |
? | |
1.14.14.108 | additional information | no activity with (-)-camphor and other ketocamphanes that do not possess a keto group at position 2 | Pseudomonas putida C1 / ATCC 17453 | ? | - |
? | |
1.14.14.155 | (-)-bornanone + FMNH2 + O2 | i.e. (-)-camphor | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | additional information | enzyme is associated with a NADH oxidase | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | additional information | rubredoxin not mentioned | Pseudomonas putida | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.108 | More | enzyme components form a very loose complex | Pseudomonas putida |
1.14.14.108 | More | enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase | Pseudomonas putida |
1.14.14.155 | monomer | 1 * 40300, oxygenating component, SDS-PAGE | Pseudomonas putida |
1.14.14.155 | More | enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase | Pseudomonas putida |
1.14.14.155 | More | enzyme components from a very loose complex | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.108 | 2,5-diketocamphane 1,2-monooxygenase | - |
Pseudomonas putida |
1.14.14.108 | 3,6-diketocamphane 1,6-monooxygenase | different enzyme with similar biochemical properties, immunological cross-reactivity | Pseudomonas putida |
1.14.14.155 | 3,6-diketocamphane 1,6-monooxygenase | different enzyme with similar biochemical properties, immunological cross-reactivity | Pseudomonas putida |
1.14.14.155 | 3,6-diketocamphane 1,6-monooxygenase | isoform, which is probably a different enzyme | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.108 | FMN | binds reversibly | Pseudomonas putida | |
1.14.14.155 | FMN | binds reversibly | Pseudomonas putida | |
1.14.14.155 | additional information | absolute specificity | Pseudomonas putida | |
1.14.14.155 | additional information | interaction of inducible NADH dehydrogenase component via bound FMN with oxygenating component | Pseudomonas putida | |
1.14.14.155 | additional information | FAD is ineffective | Pseudomonas putida | |
1.14.14.155 | additional information | purified NADH dehydrogenase component from 2,5-diketocamphane-1,2-monooxygenase can be used as electron donor | Pseudomonas putida |