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Literature summary extracted from

  • Yu, C.A.; Gunsalus, I.C.
    Monoxygenases. VII. Camphor ketolactonase I and the role of three protein components (1969), J. Biol. Chem., 244, 6149-6152.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.108 0.0004
-
 FMN component E1 Pseudomonas putida
1.14.14.108 0.001
-
 FAD component E1 Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.108 cytoplasm
-
 Pseudomonas putida 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.108 40000
-
 component E1, analytical centrifugation Pseudomonas putida
1.14.14.108 80000
-
 component E2, analytical centrifugation Pseudomonas putida
1.14.14.108 120000
-
 component E3, analytical centrifugation Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.108 (+)-bornane-2,5-dione + FMNH2 + O2 Pseudomonas putida i.e. 2,5-diketocamphane, reaction in camphor catabolism of Pseudomonas putida ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.108 Pseudomonas putida
-
 strain PpG786, mutant derived from strain PpG1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.108 separation of components E1, E2, E3 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.108 10 20 component E3, ketolactonizing Pseudomonas putida
1.14.14.108 24
-
 component E2 Pseudomonas putida
1.14.14.108 420
-
 purified component E1, FMN-coupled NADH-dehydrogenase Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.14.14.108 -20°C, ammonium sulfate precipitates as frozen pastes stable over a long period of time Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.108 (+)-5-oxo-1,2-campholide + FAD + H2O
-
 Pseudomonas putida (+)-bornane-2,5-dione + FADH2 + O2
-
 r
1.14.14.108 (+)-bornane-2,5-dione + FMNH2 + O2 i.e. 2,5-diketocamphane Pseudomonas putida 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O i.e. cyclopentenoic acid ?
1.14.14.108 (+)-bornane-2,5-dione + FMNH2 + O2 i.e. 2,5-diketocamphane, reaction in camphor catabolism of Pseudomonas putida Pseudomonas putida ?
-
 ?
1.14.14.108 additional information rubredoxin not mentioned Pseudomonas putida ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.108 30
-
 assay at Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.108 50
-
 10 min, component E2 retains full activity, component E3 loses 50%, component E3 loses 90% in the presence of substrate or component E1 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.108 20
-
 NADH component E3 Pseudomonas putida
1.14.14.108 31.7
-
 NADH component E2 Pseudomonas putida
1.14.14.108 53.3
-
 NADH component E1 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.108 7.2
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.14.108 6.5 8.5 80% of activity maximum at pH 6.5 and pH 8.5 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.108 FAD 2 mol per mol of component E3 Pseudomonas putida
1.14.14.108 FMN 0.4 mol per mol of component E1, 0.7 mol per mol of component E2 Pseudomonas putida
1.14.14.108 FMN components E1 and E2 bind FMN reversibly Pseudomonas putida