Literature summary extracted from
Conrad, H.E.; DuBus, R.; Gunsalus, I.C.
An enzyme system for cyclic ketone lactonization (1961), Biochem. Biophys. Res. Commun., 6, 293-297.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.14.14.108 |
additional information |
boiled cell-free extract, activation, replaces FMN in the assay |
Pseudomonas putida |
|
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.14.14.108 |
2,2'-bipyridine |
inhibition of the whole enzyme complex, but not of the components alone; reactivation by Fe2+, not by Co2+, Cu2+, Mn2+, Mg2+, Zn2+, Fe3+ |
Pseudomonas putida |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.14.14.108 |
cytoplasm |
- |
Pseudomonas putida |
5737 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.14.14.108 |
Fe2+ |
requirement |
Pseudomonas putida |
|
1.14.14.108 |
Fe2+ |
no Fe2+ in dehydrogenase |
Pseudomonas putida |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.14.108 |
Pseudomonas putida |
- |
wild type strain C1B (PpG1) |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.14.108 |
component E1, FMN-reductase |
Pseudomonas putida |
1.14.14.108 |
component E2, lactonizing enzyme |
Pseudomonas putida |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
1.14.14.108 |
(+)-bornane-2,5-dione + FMNH2 + O2 = (+)-5-oxo-1,2-campholide + FMN + H2O |
mechanism |
Pseudomonas putida |
|
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
1.14.14.108 |
inactive enzyme after dialysis can be reactivated by Fe2+ at 0.1 mM, Fe3+, Co2+, Zn2+, Mn2+, Mg2+, and Cu2+ are not usefull for reactivation |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.14.14.108 |
1.67 |
- |
component E2, lactonizing |
Pseudomonas putida |
1.14.14.108 |
38.3 |
- |
component E1, FMN-reductase |
Pseudomonas putida |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.14.108 |
(+)-bornane-2,5-dione + FMNH2 + O2 |
i.e. 2,5-diketocamphane |
Pseudomonas putida |
3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O |
i.e. cyclopentenoic acid |
? |
|
1.14.14.108 |
additional information |
rubredoxin not mentioned |
Pseudomonas putida |
? |
- |
? |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.14.14.108 |
30 |
- |
assay at |
Pseudomonas putida |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.14.14.108 |
7.2 |
- |
- |
Pseudomonas putida |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.14.108 |
FAD |
activation, 30% activity of FMN |
Pseudomonas putida |
|
1.14.14.108 |
FMN |
both enzymatic steps require FMN, not replaceable by GSH, Cys, sodium borohydride or ascorbate |
Pseudomonas putida |
|