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Literature summary extracted from
- Recombinant human phenylalanine hydroxylase: novel regulatory and structural properties (1996), Arch. Biochem. Biophys., 333, 85-95.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.16.1 | cAMP-dependent protein kinase | - |
Rattus norvegicus | |
| 1.14.16.1 | cAMP-dependent protein kinase | 1.5fold stimulation of recombinant enzyme | Homo sapiens | |
| 1.14.16.1 | lysolecithin | - |
Rattus norvegicus |  |
| 1.14.16.1 | lysolecithin | 2fold activation of recombinant enzyme, cofactor tetrahydrobiopterin | Homo sapiens | |
| 1.14.16.1 | phenylalanine | - |
Rattus norvegicus | |
| 1.14.16.1 | phenylalanine | recombinant enzyme, 2fold increase in activity, cofactor tetrahydrobiopterin | Homo sapiens | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.16.1 | - |
Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.16.1 | (7R)-5,6,7,8-tetrahydrobiopterin | - |
Homo sapiens | |
| 1.14.16.1 | (7R)-5,6,7,8-tetrahydropterin | 0.001 mM, 50% inhibition at 0.5 mM phenylalanine, 0.004 mM, 50% inhibition at 0.1 mM phenylalanine, recombinant enzyme | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.16.1 | 0.003 | - |
(6R)-5,6,7,8-tetrahydrobiopterin | recombinant enzyme | Homo sapiens | |
| 1.14.16.1 | 0.025 | - |
(6R)-5,6,7,8-tetrahydrobiopterin | recombinant enzyme, phenylalanine-activated | Homo sapiens | |
| 1.14.16.1 | 0.043 | - |
phenylalanine | recombinant enzyme, cofactor (6R)-methyltetrahydropterin | Homo sapiens | |
| 1.14.16.1 | 0.045 | - |
6-methyltetrahydropterin | recombinant enzyme | Homo sapiens | |
| 1.14.16.1 | 0.049 | - |
phenylalanine | phenylalanine-activated recombinant enzyme, cofactor tetrahydrobiopterin | Homo sapiens | |
| 1.14.16.1 | 0.05 | - |
phenylalanine | recombinant enzyme, cofactor tetrahydrobiopterin | Homo sapiens | |
| 1.14.16.1 | 0.055 | - |
phenylalanine | recombinant enzyme, cofactor (7R)-5,6,7,8-tetrahydrobiopterin | Homo sapiens | |
| 1.14.16.1 | 0.2 | - |
(7R)-5,6,7,8-tetrahydrobiopterin | recombinant enzyme | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.16.1 | Homo sapiens | - |
- |
- |
| 1.14.16.1 | Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.14.16.1 | side-chain modification | incorporation of 0.6 mol phosphate per enzyme subunit, recombinant enzyme | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.16.1 | ammonium sulfate, Phenyl-Sepharose, DEAE-Sepharose, recombinant enzyme | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.1 | 2.6 | - |
recombinant enzyme | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.16.1 | L-phenylalanine + (7R)-5,6,7,8-tetrahydrobiopterin + O2 | - |
Homo sapiens | L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
r | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.16.1 | 0.0015 | - |
(7R)-5,6,7,8-tetrahydrobiopterin | - |
Homo sapiens | |
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