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Literature summary extracted from
- Biochemical characterization of recombinant human phenylalanine hydroxylase produced in Escherichia coli (1987), J. Biol. Chem., 262, 2228-2233.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.16.1 | expression in Escherichia coli | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.16.1 | 4-Chlorophenylalanine | recombinant enzyme, 3 mM, almost complete inhibition | Homo sapiens |  |
| 1.14.16.1 | tryptophan | recombinant enzyme, 3 mM, approx. 75% inhibition | Homo sapiens | |
| 1.14.16.1 | tyrosine | recombinant enzyme, 3 mM, approx. 50% inhibition | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.16.1 | 0.01 | 0.015 | 6-methyltetrahydropterin | - |
Homo sapiens | |
| 1.14.16.1 | 0.016 | - |
tetrahydrobiopterin | recombinant enzyme | Homo sapiens | |
| 1.14.16.1 | 0.023 | - |
tetrahydrobiopterin | native liver enzyme in crude extract | Homo sapiens | |
| 1.14.16.1 | 0.05 | 0.075 | L-phenylalanine | recombinant enzyme, cofactor tetrahydrobiopterin | Homo sapiens | |
| 1.14.16.1 | 0.09 | - |
L-phenylalanine | native liver enzyme in crude extract, cofactor 6-methyltetrahydropterin | Homo sapiens | |
| 1.14.16.1 | 0.3 | 0.4 | L-phenylalanine | native and recombinant enzyme, cofactor 6-methyltetrahydropterin | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.16.1 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.16.1 | recombinant enzyme, partially purified | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.16.1 | L-phenylalanine + tetrahydrobiopterin + O2 | - |
Homo sapiens | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.1 | 30 | - |
native and recombinant enzyme | Homo sapiens |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.1 | 15 | 42 | native and recombinant enzyme | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.16.1 | tetrahydrobiopterin | - |
Homo sapiens | |
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