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Literature summary extracted from

  • Shiman, R.
    Purification and assay of rat liver phenylalanine 4-monooxygenase (1987), Methods Enzymol., 142, 17-27.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.16.1 lysolecithin
-
 Rattus norvegicus
1.14.16.1 additional information
-
 Rattus norvegicus
1.14.16.1 phenylalanine major regulator of liver enzyme, binds at an effector site converting the inactive to an active enzyme Rattus norvegicus
1.14.16.1 Phospholipids activate Rattus norvegicus

General Stability

EC Number General Stability Organism
1.14.16.1 frozen and thawed enzyme is 10-20% less active than before freezing Rattus norvegicus
1.14.16.1 glycerol + EDTA stabilize Rattus norvegicus
1.14.16.1 NaCl or KCl above 200 mM stabilize Rattus norvegicus
1.14.16.1 nonionic detergents e.g. Triton X-100 and Tween 80, 0.03-0.1%, stabilize Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.1 (6R)-L-erythro-tetrahydrobiopterin inhibits phenylalanine and lysolecithin activation Rattus norvegicus
1.14.16.1 catechol
-
 Rattus norvegicus
1.14.16.1 glycerol
-
 Rattus norvegicus
1.14.16.1 H2O2 inactivates the reduced form of the enzyme Rattus norvegicus
1.14.16.1 Tris
-
 Rattus norvegicus
1.14.16.1 Tween 80
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.1 0.01 0.015 tetrahydrobiopterin substrate phenylalanine Rattus norvegicus
1.14.16.1 0.045
-
 6-methyltetrahydropterin substrate phenylalanine Rattus norvegicus
1.14.16.1 0.18
-
 phenylalanine cofactor 6-methyltetrahydropterin Rattus norvegicus
1.14.16.1 0.2
-
 phenylalanine cofactor tetrahydrobiopterin Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.16.1 cytoplasm
-
 Rattus norvegicus 5737
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.1 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.16.1 side-chain modification
-
 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.1 12.5 13
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.14.16.1 -70°C, 2 years, no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 additional electron donors: 6-methylpterin, 6,7-dimethyltetrahydropterin Rattus norvegicus L-tyrosine + dihydrobiopterin + H2O
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 additional electron donors: 6-methyltetrahydropterin, 7-methylpterin, and 2,4,5-triamino-6-hydroxypyrimidine Rattus norvegicus L-tyrosine + dihydrobiopterin + H2O
-
 ?
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2 low activity with tetrahydrobiopterin can be selectively increased by a wide variety of reversible and irreversible modificators of the enzyme, e.g. interaction with phospholipids Rattus norvegicus L-tyrosine + dihydrobiopterin + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.16.1 25
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.16.1 additional information artificial cofactors: 6-methyltetrahydropterin, 6,7-dimethyltetrahydropterin Rattus norvegicus
1.14.16.1 additional information artificial cofactors: 6-methyltetrahydropterin, 7-methylpterin Rattus norvegicus
1.14.16.1 tetrahydrobiopterin
-
 Rattus norvegicus