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Literature summary extracted from

  • Hillas, P.J.; Fitzpatrick, P.F.
    A mechanism for hydroxylation by tyrosine hydroxylase based on partitioning of substituted phenylalanines (1996), Biochemistry, 35, 6969-6975.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.2 recombinant Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.16.2 L-tyrosine + a 5,6,7,8-tetrahydropteridine + O2 = L-dopa + a 4a-hydroxy-5,6,7,8-tetrahydropteridine mechanism Rattus norvegicus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 4-bromo-L-phenylalanine + 6-methyltetrahydropterin + O2 low activity Rattus norvegicus ?
-
 ?
1.14.16.2 4-chloro-L-phenylalanine + 6-methyltetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
1.14.16.2 4-fluoro-L-phenylalanine + 6-methyltetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
1.14.16.2 4-methoxy-L-phenylalanine + 6-methyltetrahydropteridine + O2
-
 Rattus norvegicus ?
-
 ?
1.14.16.2 4-methyl-L-phenylalanine + 6-methyltetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
1.14.16.2 L-phenylalanine + tetrahydropteridine + 2 O2
-
 Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydropteridine + 2 H2O
-
 ?
1.14.16.2 L-tyrosine + 6-methyltetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2
-
 Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?
1.14.16.2 additional information 4-substituted substrate analogues get hydroxylated at position 4 or 3, the latter is preferred with big-sized substituents, multiply hydroxylated products occur as well Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.16.2 25
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.16.2 7.1
-
 assay at Rattus norvegicus