Literature summary extracted from

Goodwill, K.E.; Sabatier, C.; Stevens, R.C.
Crystal structure of tyrosine hydroxylase with bound cofactor analog and iron at 2.3.ANG. resolution: self-hydroxylation of Phe300 and the pterin-binding site (1998), Biochemistry, 37, 13437-13445.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.16.2	expression in Escherichia coli	Rattus norvegicus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.14.16.2	3D-structure model, ligand binding of pterin analogues	Rattus norvegicus
1.14.16.2	crystals of the binary complex with iron and 7,8-dihydrobiopterin obtained by equilibrium dialysis, solutions degassed by helium and crystal growth in nitrogen atmosphere at 4°C	Rattus norvegicus

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.14.16.2	additional information	investigation of the role of several amino acid residues in binding of substrate and ligands by site-specific mutagenesis	Rattus norvegicus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.14.16.2	5-[(3-azido-6-nitrobenzylidene)amino]-2,6-diamino-4-pyrimidinone	competitive against tetrahydrobiopterin	Rattus norvegicus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.16.2	Rattus norvegicus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.14.16.2	recombinant from Escherichia coli	Rattus norvegicus

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
1.14.16.2	L-tyrosine + a 5,6,7,8-tetrahydropteridine + O2 = L-dopa + a 4a-hydroxy-5,6,7,8-tetrahydropteridine	stereochemical analysis of ligand binding	Rattus norvegicus	a
1.14.16.2	L-tyrosine + a 5,6,7,8-tetrahydropteridine + O2 = L-dopa + a 4a-hydroxy-5,6,7,8-tetrahydropteridine	active binding site	Rattus norvegicus	a

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.16.2	L-tyrosine + 6-methyltetrahydropterin + O2	-	Rattus norvegicus	?	-	?
1.14.16.2	L-tyrosine + tetrahydrobiopterin + O2	-	Rattus norvegicus	3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O	3,4-dihydroxy-L-phenylalanine is identical with dopa	?

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Release 2023.1 (January 2023)