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Literature summary extracted from
- Purification and some properties of tyrosine 3-monooxygenase from rat adrenal (1982), Eur. J. Biochem., 122, 49-55.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.16.2 | Dextran sulfate | activates the enzyme in the crude extract, less effective with the purified enzyme | Rattus norvegicus | |
| 1.14.16.2 | heparin | activates the enzyme in the crude extract, less effective with the purified enzyme | Rattus norvegicus |  |
| 1.14.16.2 | phosphatidyl-L-serine | no stimulation | Rattus norvegicus | |
| 1.14.16.2 | phosphatidylinositol | no stimulation | Rattus norvegicus | |
| 1.14.16.2 | Phospholipid | no stimulation | Rattus norvegicus | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.16.2 | EDTA, 0.1 mM stabilizes | Rattus norvegicus |
| 1.14.16.2 | glycerol, 25% stabilizes | Rattus norvegicus |
| 1.14.16.2 | Tween 80, 0.05% stabilizes | Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.16.2 | additional information | - |
additional information | - |
Rattus norvegicus | |
| 1.14.16.2 | 0.058 | - |
2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine | - |
Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.2 | additional information | - |
- |
Rattus norvegicus |
| 1.14.16.2 | 59000 | - |
4 * 59000, SDS-PAGE | Rattus norvegicus |
| 1.14.16.2 | 260000 | - |
gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.16.2 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.16.2 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.16.2 | adrenal gland | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.2 | 1.6 | - |
purified enzyme | Rattus norvegicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.16.2 | -80°C, 0.05% Tween 80, 0.1 mM EDTA, 25% glycerol, 5 mM sodium phosphate buffer, pH 7.5, 3 months without loss of activity | Rattus norvegicus |
| 1.14.16.2 | 4°C, 5 h, without stabilizing agents, almost complete loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.16.2 | L-tyrosine + 2-amino-4-hydroxy-6-methyltetrahydropterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
| 1.14.16.2 | L-tyrosine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.16.2 | tetramer | 4 * 59000, SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.2 | 4 | - |
5 h, without stabilizing agent, complete loss of activity | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.2 | 6 | 7.5 | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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