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Literature summary extracted from

  • Fitzpatrick, P.F.; Chlumsky, L.J.; Dauber, S.C.; O' Malley, K.L.
    Expression of rat tyrosine hydroxylase in insect tissue culture cells and purification and characterization of the cloned enzyme (1990), J. Biol. Chem., 265, 2042-2047.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.2 expression in Spodoptera frugiperda cells via baculovirus expression system Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.2 0.006
-
O2 below, recombinant enzyme Rattus norvegicus
1.14.16.2 0.0094
-
tyrosine recombinant enzyme, pH-independent Rattus norvegicus
1.14.16.2 0.021
-
Tetrahydropterin recombinant enzyme, value is pH-dependent Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.2 Iron 0.1 atom per subunit Rattus norvegicus
1.14.16.2 phosphate 1 covalently bound residue per subunit Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.2 55000
-
4 * 55000, recombinant enzyme, SDS-PAGE Rattus norvegicus
1.14.16.2 250000
-
gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.16.2 no glycoprotein
-
Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.2 recombinant enzyme from Spodoptera frugiperda Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.2 0.03 0.1 recombinant enzyme, cell lysate Rattus norvegicus
1.14.16.2 1.7
-
purified, recombinant enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2
-
Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?

Subunits

EC Number Subunits Comment Organism
1.14.16.2 tetramer 4 * 55000, recombinant enzyme, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.16.2 7
-
broad optimum, recombinant enzyme Rattus norvegicus
1.14.16.2 7.1
-
-
Rattus norvegicus