EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.16.2 | expression in Spodoptera frugiperda cells via baculovirus expression system | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.16.2 | 0.006 | - |
O2 | below, recombinant enzyme | Rattus norvegicus | |
1.14.16.2 | 0.0094 | - |
tyrosine | recombinant enzyme, pH-independent | Rattus norvegicus | |
1.14.16.2 | 0.021 | - |
Tetrahydropterin | recombinant enzyme, value is pH-dependent | Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.16.2 | Iron | 0.1 atom per subunit | Rattus norvegicus | |
1.14.16.2 | phosphate | 1 covalently bound residue per subunit | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.16.2 | 55000 | - |
4 * 55000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
1.14.16.2 | 250000 | - |
gel filtration | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.16.2 | Rattus norvegicus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.14.16.2 | no glycoprotein | - |
Rattus norvegicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.16.2 | recombinant enzyme from Spodoptera frugiperda | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.16.2 | 0.03 | 0.1 | recombinant enzyme, cell lysate | Rattus norvegicus |
1.14.16.2 | 1.7 | - |
purified, recombinant enzyme | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.16.2 | L-tyrosine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.16.2 | tetramer | 4 * 55000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.16.2 | 7 | - |
broad optimum, recombinant enzyme | Rattus norvegicus |
1.14.16.2 | 7.1 | - |
- |
Rattus norvegicus |