EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.16.2 | heparin | 0.1 mg/ml, activates | Rattus norvegicus | |
1.14.16.2 | additional information | activation by phosphorylation | Rattus norvegicus | |
1.14.16.2 | phosphatidylinositol | 0.1 mg/ml activates | Rattus norvegicus | |
1.14.16.2 | SDS | 0.01%, activates | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.16.2 | additional information | - |
additional information | phosphorylation converts the enzyme from a form possessing a high Km for pterin cofactor to a form with a low Km for pterin cofactor | Rattus norvegicus | |
1.14.16.2 | additional information | - |
additional information | rat brain: 2 kinetically distinguishable forms: low Km form, high Km form | Rattus norvegicus | |
1.14.16.2 | 0.075 | - |
tyrosine | with 6-methyltetrahydropterin | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.16.2 | cytosol | - |
Rattus norvegicus | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.16.2 | 56000 | - |
4 * 56000, about, pheochromocytoma tumor, SDS-PAGE and amino acid sequence analysis | Rattus norvegicus |
1.14.16.2 | 225000 | - |
pheochromocytoma tumors, asymmetric protein, native PAGE, gel filtration and sucrose density gradient centrifugation | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.16.2 | L-tyrosine + (6R)-L-erythro-1',2'-dihydroxypropyltetrahydropterin + O2 | Rattus norvegicus | first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine | ? | - |
? | |
1.14.16.2 | L-tyrosine + tetrahydrobiopterin + O2 | Rattus norvegicus | first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.16.2 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.16.2 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.16.2 | pheochromocytoma cell | tumor | Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.16.2 | 330 | - |
purified enzyme | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.16.2 | L-tyrosine + (6R)-L-erythro-1',2'-dihydroxypropyltetrahydropterin + O2 | first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine | Rattus norvegicus | ? | - |
? | |
1.14.16.2 | L-tyrosine + 2-amino-4-hydroxy-6,7-dimethyltetrahydropterin + O2 | synthetic pterin as electron donor | Rattus norvegicus | ? | - |
? | |
1.14.16.2 | L-tyrosine + 2-amino-4-hydroxy-6-methyltetrahydropterin + O2 | synthetic pterin as electron donor | Rattus norvegicus | ? | - |
? | |
1.14.16.2 | L-tyrosine + 6-methyltetrahydropterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
1.14.16.2 | L-tyrosine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? | |
1.14.16.2 | L-tyrosine + tetrahydrobiopterin + O2 | first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine | Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.16.2 | tetramer | 4 * 56000, about, pheochromocytoma tumor, SDS-PAGE and amino acid sequence analysis | Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.16.2 | 37 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.16.2 | 6.2 | - |
- |
Rattus norvegicus |