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Literature summary extracted from

  • Nelson, T.J.; Kaufman, S.
    Interaction of tyrosine hydroxylase with ribonucleic acid and purification with DNA-cellulose or poly(A)-sepharose affinity chromatography (1987), Arch. Biochem. Biophys., 257, 69-84.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.16.2 RNA below 0.015 mg/ml activation, rat brain enzyme contains RNA, about 10% of the total mass Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.2 RNA above 0.015 mg/ml Bos taurus
1.14.16.2 RNA above 0.015 mg/ml Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.2 additional information
-
 additional information effect of RNA on Km Rattus norvegicus
1.14.16.2 additional information
-
 additional information effect of RNA on Km Bos taurus
1.14.16.2 additional information
-
 additional information rat brain: 2 kinetically distinguishable forms: low Km form, high Km form Rattus norvegicus
1.14.16.2 0.0061
-
 tyrosine
-
 Rattus norvegicus
1.14.16.2 0.08
-
 Tetrahydropterin low Km form Rattus norvegicus
1.14.16.2 0.63
-
 Tetrahydropterin high Km form Rattus norvegicus
1.14.16.2 0.95
-
 6-Methyl-5,6,7,8-tetrahydropterin
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.2 phosphate
-
 Bos taurus
1.14.16.2 phosphate activated enzyme contains: 0.75 mol per mol of subunit Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.2 61100
-
 2 * 61100 + 2 * 62400, SDS-PAGE Bos taurus
1.14.16.2 62400
-
 2 * 61100 + 2 * 62400, SDS-PAGE Bos taurus
1.14.16.2 63300
-
 4 * 63300, SDS-PAGE Rattus norvegicus
1.14.16.2 210000 211000 gel filtration Bos taurus
1.14.16.2 239000
-
 brain, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 Rattus norvegicus rate-limiting step in catecholamine biosynthesis 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 Bos taurus rate-limiting step in catecholamine biosynthesis 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Bos taurus
-
-
-
1.14.16.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.2 2 kinetically distinguishable forms of the enzyme: low Km form and high Km form Rattus norvegicus
1.14.16.2 from adrenal medulla Bos taurus
1.14.16.2 from brain Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.2 adrenal medulla
-
 Rattus norvegicus
-
1.14.16.2 adrenal medulla
-
 Bos taurus
-
1.14.16.2 brain
-
 Rattus norvegicus
-
1.14.16.2 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.2 0.0916
-
 purified enzyme from brain Rattus norvegicus
1.14.16.2 0.18
-
 purified enzyme from adrenal medulla Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.14.16.2 -70°C, in presence of diisopropylfluorophosphate, 2 months Bos taurus
1.14.16.2 4°C, in presence of diisopropylfluorophosphate, 1 week Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 L-tyrosine + 6-methyltetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
1.14.16.2 L-tyrosine + 6-methyltetrahydropterin + O2
-
 Bos taurus ?
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2
-
 Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2
-
 Bos taurus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 rate-limiting step in catecholamine biosynthesis Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 rate-limiting step in catecholamine biosynthesis Bos taurus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.16.2 tetramer 4 * 63300, SDS-PAGE Rattus norvegicus
1.14.16.2 tetramer 2 * 61100 + 2 * 62400, SDS-PAGE Bos taurus